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Noninvasive detection of fetal health status

A health status, fetal technology, applied in the direction of microbial determination/inspection, measuring devices, instruments, etc., can solve the problems of large-scale clinical application, time-consuming, high failure rate, etc.

Active Publication Date: 2015-05-13
BGI GENOMICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although the most sensitive method of directly detecting the presence of pathogens is pathogen culture, it is very time-consuming and has a high failure rate
Therefore, large-scale clinical application of pathogen culture has not been seen

Method used

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  • Noninvasive detection of fetal health status
  • Noninvasive detection of fetal health status
  • Noninvasive detection of fetal health status

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Example 1 Determination of viruses as biomarkers for Down syndrome

[0106] This example illustrates the non-invasive genetic analysis of fetal chromosomal aneuploidy risk by obtaining peripheral blood (5mL) from pregnant women, extracting cell-free DNA, using new high-throughput technology for sequencing, and bioinformatics analysis. The exogenous sequence is related to the sequence of the host organism. The present results indicate that exogenous sequences are relevant to the health status of the host organism.

[0107] With the knowledge of the pregnant women, the researchers obtained peripheral blood samples (12-24 gestational weeks) and marked them with barcodes to facilitate information classification and sample management. Serum was isolated from the peripheral blood, and free DNA was extracted from the serum. DNA libraries are constructed and then subjected to high-throughput sequencing. The sequencing results are compared to the human genome sequence. Tho...

Embodiment 2

[0135] The sequence analysis of embodiment 2 fetal pathogens

[0136] When a pathogenic infection occurs in a pregnant female, trace amounts of said pathogenic DNA are present in the peripheral blood of said pregnant female. For example, we detected HBV DNA in the peripheral blood by DNA sequencing technology. As one of ordinary skill in the art can understand, the more DNA of the pathogen initially present in the peripheral blood of the pregnant female, the more likely the fetus is infected by the pathogen. Accordingly, provided herein is a method for detecting infection of a fetus by a pathogen using a chip containing probes for said pathogen. Pathogen-specific sequences are detected by target sequence capture followed by sequencing. A cut-off value is established based on the case-control group statistical analysis, and the cut-off value is used to determine the infection status of the fetus.

[0137] The present disclosure also relates to the design of probes that hyb...

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Abstract

The present invention discloses a method to identify biomarkers of fetal health status from foreign organism such as a virus, a bacterium, a fungus and a parasite, and uses thereof for noninvasive determination of fetal health status. And the present invention also provides a system and a kit which are used in the method.

Description

[0001] Cross Reference Related Applications [0002] The present invention claims July 31, 2012 as the filing date, the Chinese patent application number is 201210271283.4, and the title of the invention is "Method for Determining Fetal Health Status", the entire content of this application is incorporated by reference for all purposes This article. technical field [0003] The present invention relates to a non-invasive method for determining the health status of a fetus. In particular, the present invention relates to methods for detecting fetal health status by assessing biomarkers associated with exogenous organisms in biological samples from pregnant women. Background technique [0004] Routine prenatal diagnostic methods with invasive procedures, such as chorionic villus sampling and amniocentesis, carry potential risks to both the fetus and the mother. Noninvasive screening for fetal aneuploidy using maternal serum markers and ultrasound is available, but has limite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/00
CPCC12Q1/6883C12Q2600/158G01N33/5091G01N33/569
Inventor 王威李英睿徐煜刘萍孙广青曾玺
Owner BGI GENOMICS CO LTD
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