Sericin protein microneedle for drug delivery and preparation method thereof

A sericin and microneedle technology, applied in the field of biomedical engineering, can solve the problems of uncontrollable slow release process, poor biocompatibility, large trauma, etc., and achieve the effect of good application development prospect, good performance and easy preparation.

Inactive Publication Date: 2015-05-20
陶虎
View PDF4 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a biocompatibility and antibacterial properties, but also has controllable sustained release, high efficiency, low cost, easy preparation, flexibility, and natural degradation Sericin microneedles with advantages such as sericin are used to solve a series of problems in the existing drug delivery technology, such as high cost, poor biocompatibility, uncontrollable sustained release process, and large trauma.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sericin protein microneedle for drug delivery and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1): Prepare sericin solution;

[0032] Take 20 mg of sericin protein (molecular weight 5 kDa) and dissolve it in 200.00 mL of water, and make the solution uniform by magnetic stirring at a speed of 50 r / min for 0.1 h.

[0033] (2) Dissolve 0.1 g of amoxicillin in the sericin solution prepared by the above method, and make the solution uniform by magnetic stirring at a speed of 300 r / min for 1 h.

[0034] (3) Take 2mL of the amoxicillin sericin solution obtained in step (2), and slowly pour it into 4cm 2 The microneedle impression was dried at 25°C under normal pressure (0.1 MPa) for 24 hours, and cured in methanol for 1 minute.

[0035] (4) Detach the sericin microneedles from the female mold by mechanical stripping.

[0036] (5) Put the tip of the amoxicillin sericin microneedle upwards and place it horizontally to obtain the sericin egg wrapped with amoxicillin drug.

[0037] White microneedles and sericin microneedles have a length of 0.1-2 mm, a tip diameter of ...

Embodiment 2

[0040] (1): Prepare sericin solution;

[0041] Take 10 g of sericin (molecular weight 10 kDa) and dissolve it in 20.00 mL of water, and make the solution uniform by magnetic stirring at 1500 r / min for 48 h.

[0042] (2) Dissolve 0.5 g of amoxicillin in the sericin solution prepared by the above method, and make the solution uniform by magnetic stirring at a speed of 300 r / min for 1 h.

[0043] (3) Take 2mL of the amoxicillin sericin solution obtained in step (2), and slowly pour it into 4cm 2 The microneedle impression was dried at 25°C under normal pressure (0.1 MPa) for 24 hours, and cured in methanol for 60 minutes.

[0044] (4) Detach the sericin microneedles from the female mold by mechanical stripping.

[0045] (5) Put the tip of the amoxicillin sericin microneedle upwards and place it horizontally to obtain the sericin microneedle wrapped with amoxicillin drug.

[0046] (5) Gently press the amoxicillin sericin microneedle needle tip down into the patient's skin infec...

Embodiment 3

[0048] (1): Prepare sericin solution;

[0049]5 g of sericin (molecular weight 150 kDa) was dissolved in 20.00 mL of water, and the solution was homogenized by magnetic stirring at 800 r / min for 20 h.

[0050] (2) Dissolve 0.2 g of cephalosporin in the sericin solution prepared by the above method, and make the solution uniform by magnetic stirring at a speed of 300 r / min for 1 h.

[0051] (3) Take 2 mL of the cephalosporin solution obtained in step (2), and slowly pour it into 4 cm 2 Freeze-dried for 24 hours (the cold trap temperature is -55°C and the air pressure is 0.5Pa). Cured by steam annealing. The steam annealing temperature was 27 °C, the time was 48 h, and the pressure was 0.05 kPa.

[0052] (4) Detach the sericin microneedles from the female mold by mechanical stripping.

[0053] (5) Place the cephalosporin microneedle with the tip upward and place it horizontally to obtain the sericin microneedle encapsulating the cephalosporin drug.

[0054] (5) With the tip...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
diameteraaaaaaaaaa
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention relates to a sericin protein microneedle for drug delivery and a preparation method thereof, belonging to the technical field of biomedical engineering. The microneedle is prepared through the following steps: firstly, providing a sericin protein solution, dissolving the sericin protein solution in ultrapure water, carrying out magnetic stirring and dispersion, and mixing a drug required to be delivered in the sericin protein solution according to a doctor's advice, wherein the concentration of the sericin protein solution is 0.1-500 mg / mL; and then, preparing a sericin protein microneedle: filling the prepared sericin protein solution in a microneedle slot of a customized microneedle female mold, and drying and curing; and stripping the sericin protein microneedle from the microneedle female mold, so that the sericin protein microneedle is obtained. Compared with traditional microneedles, the sericin protein microneedle not only has excellent biocompatibility and antibacterial properties, but also has a series of prominent advantages of controllable slow release, high efficiency, low cost, easy preparation, flexibility, natural biodegradability, and the like, can be directly used in organisms, and has good performances under various environmental interferences, therefore, the microneedle has a good application and development prospect.

Description

technical field [0001] The invention relates to a sericin microneedle for drug delivery and a preparation method thereof, belonging to the technical field of biomedical engineering. Background technique [0002] In the field of biomedicine, humans have invented a variety of drug delivery techniques. Among them, oral administration and injection are the most commonly used administration methods, but these administration methods have many disadvantages. For example, drugs need to pass through the gastrointestinal tract, liver and other organs when administered orally. The digestion of the gastrointestinal tract and the first-pass effect of the liver often make some drugs partially or completely ineffective before reaching the target. Although injection administration can avoid the problems in the oral administration technique, it needs to pierce the deep layer of the skin with the common needle of the syringe for administration. The stimulation of the nerve cells in the deep...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K47/42A61K31/7048A61K31/43A61P31/04
Inventor 陶虎
Owner 陶虎
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap