Novel strain with azo decoloration capacity and application of novel strain

An azo dye decolorization and strain technology, which is applied to bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve rare problems and achieve the effect of low cost, wide application prospect and large processing capacity

Active Publication Date: 2015-05-20
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are only a handful of reports on the azo reduction decolorization of thermostable bacteria, and the research, development and application of thermophilic bacteria with azo dye decolorization ability are even rarer.

Method used

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  • Novel strain with azo decoloration capacity and application of novel strain
  • Novel strain with azo decoloration capacity and application of novel strain
  • Novel strain with azo decoloration capacity and application of novel strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Strain SG-1 T separation and enrichment

[0019] 1) Add 10 g of soil samples from the tropical rainforest in Xuwen County, Guangdong Province to 100 mL of 2216E medium, and incubate at 50°C for 2 weeks; dilute the culture solution in a gradient, spread it on a solid plate of 2216E medium, and carry out under the condition nourish.

[0020] 2) Pick a single colony and spread it on a new 2216E medium to further isolate and purify the bacteria.

[0021] 3) Repeat step 2) until a monoclonal colony SG-1 is obtained T .

[0022] 2. Strain SG-1 T Morphological characteristics

[0023] 1) Morphological characteristics of bacteria

[0024] Strain SG-1 T The two ends of the bacteria are blunt and round, and the bacteria are slightly curved, arranged in bamboo-like long chains (such as figure 1 shown). Strain SG-1 T It is a Gram-negative bacterium with cells about 0.4-0.5 μm wide and 0.7-2.3 μm long, without flagella, and does not produce spores (such as ...

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Abstract

The invention discloses a strain Novibacillus thermophilus CGMCC No.1.12771 and an application thereof in azo dye decoloration. The Novibacillus thermophilus strain SG-1 T is separated from soil and purified, the polyphasic taxonomy result proves that SG-1 T is a novel variety (Novibacillus thermophilus gen.nov.sp.nov.) in the taxonomy, and the novel variety (Novibacillaceae fam.nov.) is established by taking Novibacillus as a type genus. The strain SG-1 T has the effects of decolorizing azo dye golden oranges I under high-temperature anaerobic conditions by utilizing various electron donors, the decoloration rate can be 99 percent, and the strain is an effective biosystem for treating azo dye wastewater and has wide application prospects in the aspect of water environment remediation.

Description

technical field [0001] The invention relates to a new strain of bacteria and its application in environmental engineering, in particular to a strain of facultative anaerobic bacteria SG-1 T The separation and identification of and its application in the decolorization of azo dyes belong to the field of microbial system classification and environmental biotechnology. Background technique [0002] Azo dyes refer to compounds in which an azo bond (-N=N-) is used as a chromophoric group and aromatic groups are connected at both ends. Azo fuels are widely used in textile, leather, plastic, cosmetic and food industry dyeing. Because these azo dyes are toxic and refractory organic substances, have strong chemical stability, persistence and are widely distributed in the environment, azo dyes are considered to be major environmental pollutants. Even if the concentration is very low, the discharge of azo dyes into the water body will affect the light permeability of the water and th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/28C02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 杨贵芹周顺桂
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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