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Thalassiosira pseudonana and application of thalassiosira pseudonana as mercenaria mercenaria larva breeding bait

A technology of Thalassiosira pseudominiature and American clam, applied in the direction of application, microbe-based methods, microbes, etc., can solve the problems of slow reproduction speed and cannot meet the needs of rapid growth, and achieve the effect of improving stability

Inactive Publication Date: 2015-05-27
NINGBO UNIV
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  • Abstract
  • Description
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Problems solved by technology

At present, seed breeding is mostly concentrated in the southeast coast, and the main season for seedling breeding is from March to May every year. At this time, most seedling breeding areas have more cloudy and rainy weather. Microalgae, which are commonly used in shellfish seed breeding in my country, reproduce in this weather. The speed is very slow, far from meeting the bait needed for the rapid growth of this species of seedlings

Method used

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  • Thalassiosira pseudonana and application of thalassiosira pseudonana as mercenaria mercenaria larva breeding bait
  • Thalassiosira pseudonana and application of thalassiosira pseudonana as mercenaria mercenaria larva breeding bait
  • Thalassiosira pseudonana and application of thalassiosira pseudonana as mercenaria mercenaria larva breeding bait

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Embodiment 1

[0014] Embodiment 1: Separation and purification of Thalassiosira pseudonana Guh005

[0015] The seawater samples taken from the prawn breeding pond in Shanyu Village, Wengyang Town, Yueqing City, Zhejiang Province in May 2010 were picked out with a capillary tube under an inverted microscope and cultured in a 96-well plate. The culture medium used It is an f / 2 medium (Guillard and Ryther 1962, Guillard 1975), the culture temperature is 25±1°C, the light-dark ratio is 12h:12h, and it is shaken twice a day at regular intervals. Under different light intensities (100-10000Lux), a strain of marine microalgae that can reproduce normally within the range of 2000-8000Lux in the light intensity - Thalassiosira pseudonana Guh005 strain was screened out, and was released on January 18, 2015 It is preserved in the Chinese Type Culture Collection Center located in Wuhan University, Wuhan, with the preservation number: CCTCC NO.M2015044. The algal strain screened by the present invention...

Embodiment 2

[0016] Embodiment 2: the expanded cultivation of Thalassiosira pseudonana Guh005

[0017] Thalassiosira pseudonana Guh005 algae species were inoculated into a 2500mL glass Erlenmeyer flask, which contained the culture medium used for primary culture, and the formula of the culture medium was 100mg KNO per liter of seawater 3 , 20mg KH 2 PO 4 , 10mg Na 2 SiO 3 , 0.20mg MnSO 4 ·H 2 O, 5.0mg FeSO 4 ·7H 2 O. 20mg EDTA-Na 2 , 6μg VB 1 and 0.05 μg VB 12 , the salinity of seawater is 20.5, but the salinity of seawater can vary in the range of 5-30. Shake the flask 3-6 times a day during culture, and culture in natural light at (20±2)°C for 1 week to reach a density of 5000cell·μL -1 Then put it into a 50L white plastic bucket and continuously inflate it with air for secondary culture (inoculation density 40-50cell·μL -1 ), the formula for secondary culture is 50 mg KNO per liter of seawater 3 , 15mg KH 2 PO 4 , 10mg Na 2 SiO 3 , 2.50mg FeSO 4 ·7H 2 O. 10mg EDTA-Na ...

Embodiment 3

[0022] Embodiment 3: Thalassiosira pseudonana is fed to young clam clams

[0023] The planktonic larvae of Clam americana were cultured in a cement pond of 8.5×3.5×1.2m (length×width×depth) continuously filled with air. When cultivating, natural seawater with a salinity of 25 is sand-filtered and put into the pool. At a culture temperature of 22-25°C, the clams lay eggs, fertilize and hatch, and the D-shaped larvae develop in 18h-22h. At this time, golden algae are fed to maintain the golden algae in the water. Density 5-8cell·μL -1About 36 hours later, start feeding Thalassiosira pseudonana, and feed twice a day. After the first feeding, the density of Thalassiosira pseudonana should be controlled at 30cell·μL -1 Afterwards, gradually increase the feeding amount every day with the development of American clams, and keep the algae density in the water body at 2-3cell·μL before each feeding. -1 Preferably, under the temperature condition of 22-25°C, it will attach and metamor...

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Abstract

The invention provides a thalassiosira pseudonana Guh005 with the preservation number of CCTCC NO.M2015044. A specific nutrient solution formula is adopted for carrying out three-stage amplification culture on the thalassiosira pseudonana and is used for feeding after Mercenaria mercenaria D-shaped planula larva metamorphosis is finished for 36 hours, and feeding effect is the same as the conventional shell-fish bait. Under the culture by virtue of the nutrient solution formula provided by the invention, outdoor quick three-stage expanding propagation can be carried out on the marine diatom under the condition that illumination intensity is 2000-8000Lux, and especially the marine diatom can be a priority selection of a bait for breeding Mercenaria mercenaria larvae in rainy days.

Description

technical field [0001] The invention belongs to the technical field of aquaculture bait culture and feeding, and in particular relates to a strain of Thalassiosira pseudonana and its application as a bait for raising clam seedlings. Background technique [0002] American curtain clam (Mercenaria mercenaria), also known as hard shell clam, small round clam, and northern curtain clam, belongs to Lamillibranchia, Venus clam, Veneridae, Veneridae ( Venus), is a relatively large economical bivalves, the largest individual shell length is more than 100mm. The origin is the east and west coasts of the United States. It is suitable for breeding in different substrates. It grows faster and has strong disease resistance. It is the most popular in the American consumer market. Popular economical shellfish. It has been introduced into our country at the end of last century, and the scale of breeding in China's coastal areas is increasing day by day. At present, seed breeding is mostly...

Claims

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Application Information

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IPC IPC(8): C12N1/12A23K1/18A23K1/14C12R1/89
CPCC12N1/12C12N1/125C12R2001/89
Inventor 饶志鹏徐继林周成旭吴旻马斌严小军
Owner NINGBO UNIV
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