Maize tissue-specific promoter and its application in improving crop seed quality
A tissue-specific, promoter-based technology, applied in applications, plant products, angiosperms/flowering plants, etc., can solve problems such as unfavorable plant growth, breaking plant metabolic balance, and inability to effectively regulate target genes in time and space , to achieve the effect of improving plant traits and quality
Inactive Publication Date: 2017-12-15
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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Problems solved by technology
At present, most of the promoters used in plant genetic engineering are constitutive promoters, which enable high-level expression of exogenous target genes in various plant tissues and parts. However, constitutive promoters also have many disadvantages, for example, they cannot effectively Regulate the expression of target genes excessively, and excessively consume intracellular material and energy (Gittins JR, Pellny TK, Hiles ER, Rosa C, Biricolti S, et al. (2000) Transgene expression driven by heterologous ribulose-1,5-bisphosphate carboxylase / Oxygenase small-subunitgene promoters in the vegetable tissues of apple(Malus pumila mill.).Planta210:232-240.); A large number of heterologous proteins or metabolites accumulate in plants, which breaks the metabolic balance of plants and is not conducive to plant growth (Robinson DJ (1996) Environmental risk assessment of releases of transgenic plants containing virus-derived inserts. Transgenic research 5:359-362.); prone to gene silencing or co-suppression (Kumpatla SP, Chandrasekharan MB, Iyer LM, Guofu L, Hall TC (1998) Genome intruder scanning and modulation systems and transgene silencing. Trends in Plant Science 3:97-104; Mette MF, Aufsatz W, van der Winden J, Matzke MA, Matzke AJ (2000) Transcriptional silencing and promotermethylation triggered by double-stranded RNA .EMBO J 19:5194-5201.)
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Embodiment 1
[0068] Example 1 Cloning and sequence analysis of a maize tissue-specific promoter
[0069] 1. Using gene chip data to screen promoters that drive specific expression genes in maize seeds
[0070] Preparation of gene chip materials: take the roots, leaves, stems and interstems of maize B73 at the trumpet stage, mature but unpollinated young ears and filaments, embryos and A total of 14 samples, including endosperm, were set up with 3 replicates for each sample type, and the gene expression profiles of 42 samples were analyzed using a gene chip (Maize GenomeArray from Affymetrix).
[0071] Bioinformatics Analysis:
[0072] Using bioinformatics method to analyze the data of 42 samples, it was found that a gene zm.2941 (the promoter sequence of the gene is shown in SEQ ID NO.1) was specifically expressed in large quantities in the middle and late stages of embryonic development .
[0073] Table 1 Analysis of gene zm.2941 expression in different tissues by gene chip
[0074] ...
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The invention discloses a tissue-specific promoter isolated from zea mays and an application thereof to improvement of the crop seed quality, belonging to the field of isolation and application of plant tissue-specific promoters. The tissue-specific promoter with a nucleotide sequence shown in SEQ ID NO.1 is isolated from zea mays. A promoter deletion fragment which is shown in SEQ ID NO.2, SEQ ID NO.3 or SEQ ID NO.4 and still has the functions of the promoter is obtained after further truncating the sequence shown in SEQ ID NO.1. The invention also discloses a recombinant plant expression vector containing the tissue-specific promoter and a recombinant host cell. The invention further discloses the application of the tissue-specific promoter or the promoter deletion fragment to improvement of the crop seed quality and traits of crops and cultivation of new varieties of transgenic plants.
Description
technical field [0001] The present invention relates to a promoter, especially a tissue-specific promoter isolated from corn (Zea mays). The present invention also relates to a recombinant plant expression vector and a recombinant host cell containing the tissue-specific promoter. The present invention further relates to their use in The application in improving crop seed quality, improving crop traits, cultivating new plant varieties, etc. belongs to the field of separation and application of plant tissue-specific promoters. Background technique [0002] The promoter is a DNA sequence that RNA polymerase specifically recognizes and binds to, and is an important cis-acting element, generally located in the upstream region of the 5' end of the structural gene. Gene regulation in higher plants is mainly carried out at the transcription level. The promoter controls the initiation time and expression level of gene expression, and also plays a decisive role in the type of RNA pol...
Claims
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IPC IPC(8): C12N15/113C12N15/82A01H4/00A01H5/00
Inventor 王磊范云六柳小庆陈茹梅赵军周晓今田健李业
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI