Method for collecting hypophysis and hypothalamo of goat

A technology of pituitary gland and hypothalamus, which is applied in the field of collecting goat pituitary gland and hypothalamus, can solve the problems of brain tissue confusion, large mechanical damage, and long time consumption, and achieve the effect of simple method, good use effect, simple and fast operation

Inactive Publication Date: 2015-05-27
GUIZHOU UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The pituitary gland and hypothalamus obtained by this method are subject to large mechanical damage, and the brain tissue is easily mixed during the splitting process (such as the brain, hypothalamus, cerebellum, pituitary and other fragile tissues), so look for samples during the splitting process. Due to the long time-consuming, the sample is exposed to the air for a long time, resulting in serious pollution, which has devastating pollution for research such as extracting brain tissue RNA for follow-up tests

Method used

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  • Method for collecting hypophysis and hypothalamo of goat
  • Method for collecting hypophysis and hypothalamo of goat

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Embodiment Construction

[0016] The embodiment of the present invention: a method for collecting goat pituitary gland and hypothalamus, including the following steps:

[0017] 1) Take the head of the slaughtered goat, use a chainsaw or axe perpendicular to the direction of the goat’s nose, split the goat’s skull horizontally at a distance of 2-4cm from the occipital condyle in front of the ear, and cut into the bone 0.8-1.5cm, and you can see Brain tissue in the head;

[0018] 2) Split the bones on both sides along the split, separate the split part, directly expose the brain tissue, and the brain and cerebellum can be seen;

[0019] 3) Use sterile ophthalmic tweezers and ophthalmic scissors to cut out an appropriate amount of the brain, rinse the brain with DEPC treated water, wrap it in sterile tin foil, and immediately put it in liquid nitrogen for storage;

[0020] 4) Use sterile ophthalmological scissors to cut out an appropriate amount of cerebellum, rinse the cerebellum with DEPC-treated water, wrap it...

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Abstract

The invention discloses a method for collecting hypophysis and hypothalamo of a goat. By using the method, complete hypophysis and hypothalamo tissue samples with relatively low pollution degrees can be rapidly obtained after a cranial bone of the goat is split, the collecting efficiency for brain tissue samples is ensured, and defects that tissues such as hypophysis and hypothalamo are high in pollution degree and are relatively easily lost and the like in the prior art are overcome; and the method is suitable for collecting a great number of samples and is simple and rapid in operation. The method disclosed by the invention is simple and good in using effect.

Description

Technical field [0001] The invention relates to the field of life sciences, in particular to a method for collecting goat pituitary gland and hypothalamus. Background technique [0002] Goat pituitary is the most important endocrine gland of the goat body, which is divided into two parts: anterior lobe and posterior lobe. It secretes a variety of hormones, such as growth hormone, thyroid stimulating hormone, corticotropin, gonadotropin, oxytocin, prolactin, melanocyte stimulating hormone, etc., and can also store antidiuretic hormone secreted by the hypothalamus. These hormones play an important role in metabolism, growth, development and reproduction. The hypothalamus is the advanced center that regulates visceral activity under the cerebral cortex. It links visceral activity with other physiological activities and regulates important physiological functions such as body temperature, food intake, water balance, blood sugar and endocrine gland activity. Collecting intact and un...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/04G01N1/10
Inventor 陈祥冯文武龙威海许厚强李鹏程孙振梅丁玫陈伟
Owner GUIZHOU UNIV
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