Kit and detection method for detecting porcine reproductive and respiratory syndrome virus
A technology for respiratory syndrome and pig breeding, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of high requirements for experimental sites and experimental personnel, long time-consuming, virus isolation and poor sensitivity of immunofluorescence tests, etc. Achieving improved availability and reliability, improved monitoring levels, and increased consistency
A technology for respiratory syndrome and pig breeding, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of high requirements for experimental sites and experimental personnel, long time-consuming, virus isolation and poor sensitivity of immunofluorescence tests, etc. Achieving improved availability and reliability, improved monitoring levels, and increased consistency
CN104655841AInactive Publication Date: 2015-05-27SHENZHEN COMBINED BIOTECH
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[0026] Preferred embodiments of the present invention are described in detail below.
[0027] Such as figure 1 As shown, the present invention provides an ELISA kit for detecting porcine reproductive and respiratory syndrome virus, in which an enzyme-linked reaction plate 101 and various reagents 102 are arranged in the box, and various reagents specifically include enzyme-labeled conjugates, concentrated Washing solution, substrate solution, chromogenic solution, concentrated sample dilution solution, stop solution, etc.; wherein, the enzyme-linked reaction plate is an enzyme prepared by coating an antibody against porcine reproductive and respiratory syndrome virus N protein Linked reaction plate; the enzyme-labeled conjugate is an enzyme-labeled conjugate of an antibody against porcine reproductive and respiratory syndrome virus N protein labeled with horseradish peroxidase.
[0028] The enzyme-linked reaction plate and various reagents of the kit of the present invention ...
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The invention discloses a kit and a detection method for a detecting porcine reproductive and respiratory syndrome virus. An enzyme-linked reaction plate, an enzyme label conjugate, a concentration washing solution, a substrate solution, a developing solution, a concentrated sample diluents, and a stop buffer are arranged in a kit body; the kit is characterized in that the enzyme-linked reaction plate coats a polyclonal antibody of porcine reproductive and respiratory syndrome virus N protein; the enzyme label conjugate is an enzyme label conjugate of the polyclonal antibody of the porcine reproductive and respiratory syndrome virus N protein and is marked by horseradish peroxidase. By means of the sensitivity, specificity, practicability and economical efficiency of a rapid immune detection technology, the kit disclosed by the invention mainly has the advantages of being simple, convenient and fast to operate, and objective and reliable in result judgment, and as a convenient early screening tool, the sensitivity of the kit can reach 90% of the gold standard method PCR.
Description
technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit and a detection method for detecting porcine reproductive and respiratory syndrome virus. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), also known as porcine blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (porcine reproductive and respiratory syndrome virus, PRRSV). Anorexia and reproductive disorders such as abortion, stillbirth, mummified fetuses, weak piglets, and respiratory symptoms and high mortality in piglets are characterized. After the disease first broke out in the United States in 1987, it quickly spread to all pig-raising countries and regions in the world, causing huge economic losses to the pig industry. Therefore, establishing a quick and easy method to detect the virus has become an urgent need for the detection of the disease. [0003] Existing detection methods includ...
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Patent Timeline
27 May 2015
Publication
CN104655841A
- IPC
- G01N33/569; G01N33/531
- CPC
- G01N33/68; G01N33/531; G01N33/56983
- Inventors
- 邓凤林; 胡绍良