Kit and detection method for detecting porcine reproductive and respiratory syndrome virus

Abstract

The invention discloses a kit and a detection method for a detecting porcine reproductive and respiratory syndrome virus. An enzyme-linked reaction plate, an enzyme label conjugate, a concentration washing solution, a substrate solution, a developing solution, a concentrated sample diluents, and a stop buffer are arranged in a kit body; the kit is characterized in that the enzyme-linked reaction plate coats a polyclonal antibody of porcine reproductive and respiratory syndrome virus N protein; the enzyme label conjugate is an enzyme label conjugate of the polyclonal antibody of the porcine reproductive and respiratory syndrome virus N protein and is marked by horseradish peroxidase. By means of the sensitivity, specificity, practicability and economical efficiency of a rapid immune detection technology, the kit disclosed by the invention mainly has the advantages of being simple, convenient and fast to operate, and objective and reliable in result judgment, and as a convenient early screening tool, the sensitivity of the kit can reach 90% of the gold standard method PCR.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit and a detection method for detecting porcine reproductive and respiratory syndrome virus. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), also known as porcine blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (porcine reproductive and respiratory syndrome virus, PRRSV). Anorexia and reproductive disorders such as abortion, stillbirth, mummified fetuses, weak piglets, and respiratory symptoms and high mortality in piglets are characterized. After the disease first broke out in the United States in 1987, it quickly spread to all pig-raising countries and regions in the world, causing huge economic losses to the pig industry. Therefore, establishing a quick and easy method to detect the virus has become an urgent need for the detection of the disease. [0003] Existing detection methods includ...

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Problems solved by technology

[0003] Existing detection methods include virus isolation and identification, immunofluorescence test, PCR and other conventional techniques, but virus isolation and immunofluorescence test have disadvantages such as poor sensitivity and long time-consuming; High, not suitable for grassroots units to carry out work

[0004] Therefore, the prior art does not have effective tools for detecting porcine reproductive and respiratory syndrome virus

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