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Panax notoginseng root tuber production place identification method based on stable isotope fingerprints

A stable isotope and notoginseng technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of research and application, and achieve the effect of strong operability, simple method, and few detection indicators

Inactive Publication Date: 2015-06-03
YUNNAN AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

Lack of research and application of stable isotope fingerprint identification technology for traditional Chinese medicinal materials

Method used

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  • Panax notoginseng root tuber production place identification method based on stable isotope fingerprints
  • Panax notoginseng root tuber production place identification method based on stable isotope fingerprints

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0021] 1) Sample collection

[0022] From October to November 2011, three-year-old spring notoginseng were randomly selected in Jingxi, Guangxi, main notoginseng production counties of Wenshan Prefecture, Yunnan, and newly expanded production areas of notoginseng in Yunnan (Honghe, Kunming, Qujing, Xuanwei, Yuxi, Baoshan). Growers randomly collected 15-20 roots of Panax notoginseng from the east, south, west, north, and center of their planting plots or areas as a sample; 5 samples were collected from Guangxi, 17 samples from Wenshan, and 17 samples from the newly expanded area of ​​Yunnan. 22 samples were collected.

[0023] 2) Drying and crushing of samples

[0024] Wash off the root soil of Panax notoginseng with tap water + detergent, then rinse with tap water, and finally rinse repeatedly with distilled water, kill green at 105°C for 30 minutes, dry at 60°C to 70°C to constant weight, and crush the Panax notoginseng sample with a universal stainless steel grinder The pa...

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Abstract

The invention relates to a method for identifying a panax notoginseng root tuber production place based on stable isotope fingerprints, and belongs to the field of production place tracing of authentic Chinese medicinal materials. The method comprises steps as follows: (1), three-year spring panax notoginseng root tuber samples of any one place of Baise of Guangxi province, Wenshan of Yunnan province and a newly expanded area of Yunnan province are collected; (2), the collected three-year spring panax notoginseng root tuber samples are washed, dried and smashed; (3), rates of panax notoginseng powder stable isotopes delta <2>H, delta<18>O, delta <13>C and delta <15>N are detected, and the unit is permillage; numerical values of Y(Baise district of Guangxi province), Y(Wenshan district of Yunnan province) and Y(newly expanded district of Yunnan province) can be obtained according to discrimination models (1), (2) and (3), and a panax notoginseng sample comes from the region with the maximum numerical value. A producing place discrimination model is built according to stable isotope fingerprint characteristics of four elements of C, H, O and N from start of stable isotope fingerprint difference of panax notoginseng, a few test indexes are used, the accuracy is high, uncertainty based on labels and subjective judgment is reduced, and the overall discrimination rate of sources of three-year spring panax notoginseng production places can be higher than 84%.

Description

technical field [0001] The invention belongs to the field of origin traceability of authentic Chinese medicinal materials, and in particular relates to a method for identifying the origin of Panax notoginseng root based on stable isotope fingerprints. Background technique [0002] Radix Notoginseng [Panax notoginseng (Burk) F.H.Chen] is a perennial herb of Araliaceae, and its crude drug is called Radix Notoginseng, sweet in taste, slightly bitter, and warm in nature. Panax notoginseng has the functions of hemostasis, promoting blood circulation and removing blood stasis, anti-fatigue, anti-aging, hypoxia resistance, lowering blood sugar and improving the body's immunity. Cardiovascular system, central nervous system, metabolic system and other fields. Panax notoginseng originated in the Tertiary paleotropical mountainous area of ​​southwestern China 25 million years ago. Its distribution is mainly limited to the middle and high altitude areas around 23°30′N, and its distrib...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 何忠俊梁社往李龙根
Owner YUNNAN AGRICULTURAL UNIVERSITY
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