Method for inhibiting HIV-1 infectious agent from infecting primary lymphocyte by utilizing CRISPR/Cas9

A HIV-1, lymphocyte technology, applied in the field of genetic engineering and antiviral infection, can solve the problem of difficult to transduce hematopoietic cells
CN104694573AActive Publication Date: 2015-06-10WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
Publication Date
2015-06-10

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Abstract

The invention discloses a method for inhibiting HIV-1 infectious agent from infecting primary lymphocyte by utilizing CRISPR / Cas9 and relates to genetic engineering and an anti-virus infection technology. According to the method, by utilizing the latest CRISPR / Cas9 nuclease system, through the combination of Ad5F35 mosaic type adenovirus vectors of efficient targeted primary T lymphocyte, by editing CCR5 expression of human CD4+T lymphocyte, HIV-1 virus infection is effectively inhibited. According to the method, the CRISPR / Cas9 system is packaged by utilizing the Ad5F35 type adenovirus vectors, the advantages of the CRISPR / Cas9 system and the Ad5F35 type adenovirus vectors are organically combined, the novel method for resisting HIV-1 virus infection is provided, and the method has the potential for being applied for HIV-1 gene treatment research; meanwhile, the Ad5F35 type adenovirus vectors carrying with the CRISPR / Cas9 system can be used for conducting targeted editing to other genes in the primary lymphocyte or gene editing research of other hematopoietic system cells.
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Description

technical field

[0001] The invention relates to genetic engineering and anti-virus infection technology, in particular to a method for inhibiting HIV-1 from infecting primary lymphocytes by using CRISPR / Cas9. Specifically, the present invention uses Ad5F35 recombinant adenovirus expressing CRISPR / Cas9 nuclease system to target edit human primary CD4 + The HIV-1 co-receptor CCR5 on the surface of T lymphocytes destroys the CCR5 expression box or makes CCR5 lose its receptor function, thereby inhibiting HIV-1 virus infection. Background technique

[0002] Since it was first reported in 1984 that HIV-1 was the cause of AIDS (AIDS), a large number of top scientists around the world have devoted themselves to overcoming this disease. Ways such as therapy have significantly improved the quality of life of HIV-1 infected patients and greatly reduced the number of new HIV-1 infections; however, there is still a lack of effective vaccines and therapeutics that can cure HIV-1 infecti...

Claims

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