A process for separating and extracting collagen polypeptides

A collagen polypeptide and process technology, which is applied in the field of comprehensive utilization of fish waste, separation and extraction of collagen polypeptide, can solve the problem of high cost of pet food, and achieve the effects of high utilization rate, good product quality and convenient operation

Inactive Publication Date: 2015-06-10
于洪洲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the cost of pet food made in this way must be high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0014] Example 1: Wash and drain the fish skin first, then add 8 times softened water (w / w), adjust the pH to 4.7, stir and extract at 92°C for 4 hours, coarse filter; adjust the pH to 8.3, add kaolin to 65 Fine filtration at ℃, the fine filtrate is single-helix fish skin collagen; when the fine filtrate is concentrated to 1 / 3 under reduced pressure, add n-hexane or Petroleum ether or diethyl ether, stir for 1 hour, stand and extract for 6-8 hours; adjust the pH of the lower layer solution to 6.8, and adjust the temperature to 51-53°C; according to fresh fish skin: high-efficiency enzyme mixture = 1:0.002 (W / W) The proportion of high-efficiency enzyme mixture is added to the one-step enzymatic digestion for 3-5 hours, and at the same time, it is continuously stirred; Stir and decolorize at 65°C for 0.5 hours, filter, desalt the filtrate through nanofiltration membrane, concentrate in vacuum, and spray dry to obtain colorless, odorless, fat-free, low-salt and uniform molecular ...

Embodiment 2

[0015] Example 2: Use a bone and meat separator to separate the fish fillets first, add demineralized water to the fish, adjust the temperature to 50°C, add papain according to fresh fish: neutral protease = 1:0.002 (w / w), and keep stirring Enzymolysis was carried out for 3 hours; the enzymolysis solution was deodorized and spray-dried to obtain fish meat hydrolyzed protein powder; the fish bones were transferred to fish heads and fish viscera for treatment, which was Example 3.

Embodiment 3

[0016] Example 3: Dry the fish head, fish viscera together with the fish bones separated in Example 2 at 95-100°C, crush them, and pass through a 40-mesh sieve to obtain the main raw material of pet food, which is used to compound into pet food .

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PUM

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Abstract

The invention relates to a novel process for extracting collagen polypeptides from fish waste by high-valued and clean process steps. The process includes steps of extracting single-helix collagens, removing fishy smell, deodorizing, performing digestion, decoloring, desalting and spray-drying. In detail, the process includes: cleaning fish skin containing natural collagens having triple-helix tight structures, draining off, adding softened water, extracting for 3 h under controlled pH and temperature conditions, adjusting the pH value, adding diatomite, performing coarse filtration to obtain coarse filtrate that is single-helix fish skin collagens, subjecting the coarse filtrate to vacuum concentration, adding an organic solvent, mixing and stirring for 1 h, allowing the mixture to stand, extracting, performing fishy-smell removing treatment and deodorizing treatment, adding an enzyme mixture agent to perform digestion, adding a decoloring agent, stirring for decoloring for 0.5 h while controlling temperature, filtering, desalting, and performing spray-drying to obtain a white fish skin collagen polypeptide product. The process is simple in steps, convenient in operation control, high in utilization rate and good in production quality.

Description

Technical field: [0001] The invention belongs to the technical field of extraction of marine natural products, and relates to a new process for realizing comprehensive utilization of fish waste, separation and extraction of collagen polypeptides through high-value and clean process steps as raw materials. Background technique: [0002] my country is rich in aquatic product resources, accounting for 36% of the world's total aquatic products. There are currently more than 7,000 aquatic product processing enterprises in China, but most of them are only primary processing. About 57% of the waste is left after the fish is exported, and the waste is simply used as feed or fertilizer, but lacks comprehensive utilization. However, there is growing interest in deriving high-value health-enhancing products from seafood. The aquaculture processing industry produces a large amount of marine fish wastes such as fish bones, skins, fins and scales every year. Although these wastes have hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 于洪洲
Owner 于洪洲
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