Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

The application of the spider aromatic iridoid part in the preparation of n-type calcium channel inhibitors

A technology of aromatic iridoid and spider, which is applied to the application field of spider iridoid part in the preparation of N-type calcium channel inhibitor, can solve the problem that the research on spider incense is still insufficient, and achieves broad application prospects. Effect

Active Publication Date: 2018-08-17
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these previous research results have not carried out research on the specific mechanism of action of spider fragrance and related diseases, and there are fewer reports on the hydrolysis reaction of the active part of spider fragrance and the active part.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • The application of the spider aromatic iridoid part in the preparation of n-type calcium channel inhibitors
  • The application of the spider aromatic iridoid part in the preparation of n-type calcium channel inhibitors
  • The application of the spider aromatic iridoid part in the preparation of n-type calcium channel inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example provides a method for preparing the iridoid part of Aranthus chinensis, the steps are as follows: Take 1000 grams of medicinal material powder of Arachnis alba, add 3500 ml of 95% ethanol and place it in the reflux device for reflux for 3 hours, filter out the ethanol liquid after cooling, and add the residue to 3000ml of ethanol was refluxed again for 2 hours, the ethanol extract was filtered out and combined into the first ethanol filtrate to obtain about 6000ml of ethanol extract, concentrated ethanol extract to water suspension, and ethyl acetate was added to extract the iridoid part of spider , the spider iridoid part can effectively inhibit N type calcium channel. The iridoid part of the spider fragrance is added with appropriate excipients, binders, and disintegrants and compressed into tablets to obtain 1000 tablets.

Embodiment 2

[0028] This example provides a method for obtaining the hydrolyzate of the iridoid part, and the specific steps are as follows: add 1000 ml of 0.01M hydrochloric acid aqueous solution to the part of the iridoid obtained in the above-mentioned Example 1, and then rotate and stir for 1 hour After the reaction is stopped, it is neutralized with 0.01M NaOH to pH=7, then extracted with ethyl acetate, and concentrated to obtain the hydrolyzate of the iridoid part, which can effectively inhibit the N type calcium channel.

Embodiment 3

[0030] Identification and verification of the active substance of the iridoid part of spider fragrance in Example 1

[0031] The spider iridoid part obtained in the above-mentioned embodiment 1, get 50g and use 100g silica gel (200-300 mesh) to mix the sample, put 10 times the amount of silica gel column (0.5kg, 200-300 mesh), mobile phase is petroleum ether: Acetone=50:1~0:1, 7 components (Fr1-7) were eluted. Fraction 4 (Fr.4, 2.8g) was further subjected to silica gel column chromatography (petroleum ether: acetone = 20:1~1:1 gradient elution) to obtain 4 fractions (sub Fr.4.1~4.4). Wherein the second subcomponent (sub Fr.4.2, 0.8 g) is subjected to MCI gel (60%~90% methanol gradient elution), gel sephadex LH-20 (methanol), reverse phase ODS (methanol: water=50 :50~90:10) was purified to obtain compound valeriandoid B8 (compound 1, as shown in formula II, 5 mg). Component 6 (Fr.6, 5.3 g) was obtained by an approximate method to obtain valtrate hydrine J (compound 2, represe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of a valeriana jatamansi Jones part in preparation of an N-type calcium channel inhibitor and belongs to the field of medicine. The effect of the valeriana jatamansi Jones part in the N-type calcium channel inhibition is provided and two iridoid components of the valeriana jatamansi Jones part for playing a role in the N-type calcium channel inhibition are verified. The inhibition effect of the acidic hydrolysis product of the iridoid in the N-type calcium channel is firstly found. The valeriana jatamansi Jones part provided by the invention taken as a novel N-type calcium channel inhibitor has a wide application prospect and can be applied to the healthcare and treatment of the related diseases such as pain.

Description

Technical field: [0001] The invention belongs to the field of medicines, and in particular relates to the application of a spider iridoid part in the preparation of N-type calcium channel inhibitors. Background technique: [0002] N-type voltage-dependent calcium channels are mainly distributed in neuronal synaptic terminals of pain transmission and regulation pathways, participate in the release and regulation of pain mediators (such as glutamate and substance P), and play an important role in the process of pain transmission and regulation (Foreign Medicine and Pharmacy Volume, 34(2), 93-98). Therefore, blockade of N-type calcium channels can produce analgesic effects, especially in chronic pain and neuropathic pain. Although the use of opioids can relieve pain, there are side effects such as respiratory depression, constipation and addiction. Therefore, finding new analgesic drugs with high analgesic activity and few side effects has increasingly become a topic of conce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61P29/00A61P25/04A23L33/10
Inventor 胡江苗杨建年寅董发武姜河海周俊杨柳
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com