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GC-MS technology-based method for identifying fermented cordyceps sinensis mycelium varieties

A technology for fermenting Cordyceps sinensis and mycelium, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of unreported varieties of fermented cordyceps sinensis mycelium, less research on volatile components in cordyceps sinensis mycelium, etc. The effect of high sensitivity and improved accuracy

Inactive Publication Date: 2015-06-24
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The components of fermented Cordyceps mycelia mainly include cordycepin, adenosine, cordyceps polysaccharides, cordycepic acid, ergosterol, fatty acids, amino acids, etc. Previous research work also mainly focused on nucleosides, polysaccharides and non-volatile components. The research of volatile component in the silk is less, and the research that adopts GC-MS technique to distinguish fermented Cordyceps mycelia kind involved in the present invention has not yet reported

Method used

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  • GC-MS technology-based method for identifying fermented cordyceps sinensis mycelium varieties
  • GC-MS technology-based method for identifying fermented cordyceps sinensis mycelium varieties
  • GC-MS technology-based method for identifying fermented cordyceps sinensis mycelium varieties

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Embodiment 1

[0033] The present embodiment takes the detection of volatile components in a fermented Cordyceps hyphae capsule as an example to illustrate its GC-MS extraction, analysis and identification process, but the scope of protection of the present invention is not limited to this experiment, and the specific steps are as follows:

[0034] 1. Instruments and reagents

[0035] Agilent 7890A-5975C Gas Chromatography-Mass Spectrometry with Autosampler; Fermented Cordyceps Mycelia Capsules, 0.2g*60 capsules.

[0036] 2. Extraction of volatile components of fermented Cordyceps products

[0037]Accurately weigh about 10g of the fermented Cordyceps product, put it in a 500ml round bottom flask, add 250ml of deionized water, and take another 50ml of anhydrous ether as the extraction solvent, put it in a 250ml round bottom flask, two flasks On the SDE simultaneous distillation and extraction device, set the heating mantle to heat the deionized water to boil, heat the extraction solvent with...

Embodiment 2

[0053] The present embodiment takes 8 kinds of fermented Cordyceps mycelia GC-MS measurement and chemometric data analysis as an example to illustrate the identification method of fermented Cordyceps mycelia species, but the scope of protection of the present invention is not limited to this experiment, and the specific steps are as follows:

[0054] 1. Instruments and reagents

[0055] Agilent 7890A-5975C gas chromatography-mass spectrometry with automatic sampler; 5 kinds of fermented Cordyceps mycelium products with Weijian brand name (three batches each) and 1 batch of 3 fermented Cordyceps mycelia products without Weijian brand name (online shopping).

[0056] 2. Extraction of volatile components of fermented Cordyceps products

[0057] Precisely weigh about 20 g each of 8 kinds of fermented Cordyceps mycelia products, and extract them respectively. The extraction method is as follows: put the fermented Cordyceps mycelium in a 500ml round-bottomed flask, add 250ml of de...

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Abstract

The invention provides a GC-MS technology-based method for identifying fermented cordyceps sinensis mycelium varieties. The method specifically comprises the following steps: (1) extracting volatile components in fermented cordyceps sinensis mycelia; (2) determining conditions for performing GC-MS analysis on the volatile components in the fermented cordyceps sinensis mycelia and measuring samples; (3) performing qualitative analysis on volatile chemical components; and (4) performing GC-MS data analysis by virtue of a chemometrics method, and identifying the varieties of the fermented cordyceps sinensis mycelia. The method provided by the invention has the advantages that the volatile components in the fermented cordyceps sinensis mycelia are identified by selecting a GC-MS technology so as to ensure that the problem of identifying the varieties of the fermented cordyceps sinensis mycelia can be solved; and the method is easy and convenient to operate and intuitive and reliable in result, and ensures that a method for performing quality control and evaluation on fermented cordyceps sinensis mycelium products can be provided.

Description

【Technical field】 [0001] The invention relates to the field of drug detection, in particular to a method for identifying fermented Cordyceps hyphae varieties based on GC-MS technology. 【Background technique】 [0002] Cordyceps sinensis is a compound of the subunit and larva corpse of Cordyceps sinensis Sacc., a fungus of the ergot family Fungus Cordyceps sinensis Sacc., which has the functions of nourishing the lungs and kidneys, secreting essence and nourishing qi, etc. Chinese medicinal materials. Modern pharmacological research also shows that Cordyceps sinensis is beneficial to the immune system, circulatory system, cardiovascular system, and respiratory system of the human body. However, wild Cordyceps sinensis mainly exists in the alpine plateau meadows at an altitude of 3500-5000m in Qinghai, Sichuan, Tibet, Guizhou, Yunnan and other provinces in my country. The limitation of this growth environment and the crazy digging of people make the resources of natural Cordyc...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 胡坪李亚慧章弘扬张敏王月荣
Owner EAST CHINA UNIV OF SCI & TECH