Nanometer artificial seed of Dendrobium officinale and manufacturing method thereof

An artificial seed, Dendrobium candidum technology, applied in the field of medicine and biology, can solve the problems of difficult seedling cultivation, lack of endosperm nutrition, low reproduction rate, etc., achieve high germination rate and seedling rate, ensure integrity, and short reproduction cycle Effect

Active Publication Date: 2015-07-01
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The seeds of Dendrobium officinale are as fine as powder and lack endosperm nutrition. Under natural cond

Method used

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  • Nanometer artificial seed of Dendrobium officinale and manufacturing method thereof
  • Nanometer artificial seed of Dendrobium officinale and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of preparation method of dendrobium candidum nano artificial seed, comprises the steps:

[0036] (1) Induction of protocorms:

[0037]Select mature and uncracked Dendrobium officinale capsules, wash them with rice detergent for 3-5 minutes, wash them with tap water for 5-8 minutes, and then disinfect them with 75% alcohol for 30 seconds in an ultra-clean workbench, and wash them twice with sterile water , Then immerse in 0.1% mercuric chloride solution, shake and disinfect for 15 minutes, wash with sterile water for 4-5 times, dry the surface with sterile paper towels, and set aside. On the ultra-clean workbench, cut the sterilized capsule in half with a scalpel, clamp one end of the capsule with tweezers and shake gently, spread the seeds evenly on the MS medium with 10% coconut milk, and cover the bottle Cover and cultivate in the cultivation room to obtain the protocorm;

[0038] (2) Proliferation of protocorms:

[0039] Select protocorms with basically the...

Embodiment 2

[0044] Germination rate and seedling rate evaluation of embodiment 2 artificial seeds

[0045] 1. Purpose of the experiment

[0046] By comparative analysis, evaluate the germination rate and the seedling rate of the artificial seeds prepared in Example 1.

[0047] 2. Experimental method

[0048] The germination rate and seedling rate are used as indicators for examining artificial seeds (usually the germination rate of artificial seeds is assessed, but the seedling rate is the key indicator to determine whether the artificial seed production is successful).

[0049] (1) Compare the effects of different production methods on the appearance, germination rate and seedling rate of artificial seeds

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Abstract

The invention discloses a nanometer artificial seed of Dendrobium officinale and a manufacturing method thereof. The manufacturing method comprises the following steps: induction and propagation of protocorm of Dendrobium officinale; and manufacturing and germination of artificial seeds. According to the invention, nanometer technology and cell engineering technology are combined together to prepare composite endosperm and artificial seed coat of the artificial seed of Dendrobium officinale, so permeability and water retention performance of traditional embedding are improved and the growth of the protocorm is promoted; thus, the germination rate and the seedling rate of the artificial seeds are increased, and technical support is provided for practicability of germplasm breeding and industrialization development of Dendrobium officinale.

Description

technical field [0001] The invention relates to the technical field of medicine and biology, in particular to a dendrobium officinale nano-artificial seed and a preparation method thereof. Background technique [0002] Dendrobium officinal Kimura et Migo is a perennial epiphytic herbaceous plant of the genus Dendrobium orchidaceae. It is also named black knot grass because of its dark brown stem nodes. Dendrobium officinale is a traditional precious Chinese herbal medicine in my country. The 2010 edition of Pharmacopoeia of the People's Republic of China includes Dendrobium officinale derived from three species of Dendrobium. Modern pharmacological studies have shown that Dendrobium officinale contains polysaccharides, alkaloids, amino acids and other components, which have anti-tumor, anti-aging, enhancing body immunity, dilating blood vessels and anti-platelet aggregation, etc., so it is widely used in clinical and traditional Chinese medicine compound. Dendrobium officin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张桂芳赖小平关杰敏
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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