Blood cell detection reagent, blood cell processing method and blood cell identification method

A technology for detecting reagents and blood cells, which is applied in the field of blood cell detection, can solve the problems of red blood cell debris interference, white blood cells destroying the internal morphological structure information of white blood cells, no simultaneous detection of immature white blood cells, white blood cells and reticulocytes, etc., to achieve increased membrane permeability Effect

Inactive Publication Date: 2015-07-01
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all need to dissolve red blood cells and are easily disturbed by red blood cell fragments. At the same time, the damage to white blood cells destroys the internal morphological structure information of white blood cells.
[0005] In summary, there is no report on the simultaneous detection of immature white blood cells, white blood cells and reticulocytes in the current detection methods

Method used

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  • Blood cell detection reagent, blood cell processing method and blood cell identification method
  • Blood cell detection reagent, blood cell processing method and blood cell identification method
  • Blood cell detection reagent, blood cell processing method and blood cell identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Prepare blood cell detection reagent A with the following composition:

[0067]

[0068] The pH is 7.5 and the osmolarity is 642 mOsm / kg.

[0069] Add 4 μL of fresh anticoagulated blood (the anticoagulated blood has been checked by traditional manual microscopy, and the blood sample contains reticulocytes and immature white blood cells) into the above 1mL detection reagent A, and mix them separately under the condition that the temperature is kept at 42°C After incubation for 3.6 seconds, 12.6 seconds, 32.6 seconds and 62.6 seconds, the cells were detected by laser flow cytometry (excitation wavelength 633-635 nm).

[0070] The fluorescence intensity information of the cells is measured by measuring the side fluorescence at an angle of 90 degrees, and the forward scattered light intensity information of the cells is measured by measuring the forward scattered light at a low angle of 1-10 degrees. The two-dimensional scatter diagram formed by the forward scattered li...

Embodiment 2

[0072] Prepare blood cell detection reagent B with the following composition:

[0073]

[0074] The pH is 7.9 and the osmolarity is 229 mOsm / kg.

[0075] Add 4 μL of fresh anticoagulated blood (the anticoagulated blood has been inspected by traditional manual microscope, and the blood sample contains reticulocytes) into the above 1mL detection reagent B, keep the temperature at 40°C, mix for 30 seconds, and use a laser Cells were detected by flow cytometry (excitation wavelength 633-635nm).

[0076] Measure the fluorescence intensity information of the cells by measuring the side fluorescence with a measurement angle of 90 degrees, measure the forward scattered light intensity information of the cells by measuring the forward low angle 1-10 degree scattered light, and use the side scattered light with a measurement angle of 90 degrees Measure the side-scattered light intensity information of the cells.

[0077] On the two-dimensional scatter diagram formed by forward scat...

Embodiment 3

[0080] Prepare blood cell detection reagent C with the following composition:

[0081]

[0082] The pH was 9.1 and the osmolarity was 274 mOsm / kg.

[0083] Add 4 μL of fresh anticoagulated blood (the anticoagulated blood has been inspected by traditional manual microscopy, and the blood sample contains immature granulocytes) into the above 1mL detection reagent C, keep the temperature at 42°C, mix for 27 seconds, and use a laser Cells were detected by flow cytometry (excitation wavelength 633-635nm).

[0084]Measure the fluorescence intensity information of the cells by measuring the side fluorescence with a measurement angle of 90 degrees, measure the forward scattered light intensity information of the cells by measuring the forward low angle 1-10 degree scattered light, and use the side scattered light with a measurement angle of 90 degrees Measure the side-scattered light intensity information of the cells. The two-dimensional scatter point formed by the intensity of ...

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Abstract

The invention relates to the blood cell detection field and discloses a blood cell detection reagent, a blood cell processing method and a blood cell identification method. The blood cell detection reagent comprises fluorescent dye, a sphericized component and organic alcohol, wherein the fluorescent dye has cell permeability and can specially stain the nucleic acid substance in the cell; the sphericized component can sphericize red blood cells, keep the red cell membrane to be intact and cannot damage the internal structure of the white blood cell; the organic alcohol can enhance the cell permeability and aids the fluorescent dye to enter the cells. The invention also discloses a blood cell processing method and a blood cell identification method. According to the blood cell detection reagent and the method, one kind of reagent can detect different blood cells at one time, and can particularly detect juvenile white blood cells and reticulated red blood cells simultaneously, therefore, the detection cost is greatly saved, the detection speed is increased and the instrument complexity is reduced.

Description

technical field [0001] The present application relates to the field of blood cell detection, in particular to a blood cell detection reagent and a method for processing and identifying blood cells using the reagent. Background technique [0002] Blood cells in peripheral blood are formed by the proliferation, differentiation, and development of hematopoietic stem cells. Blood cells gradually mature and enter the peripheral blood to perform specific functions: Reticulocytes (RET) are immature red blood cells. Immature white blood cells refer to white blood cells that have not yet completed differentiation and development (immature white blood cells are also called immature white blood cells, mainly including blast cells (Blast), immature granulocytes (IG), immature lymphocytes, etc.). Clinically, it is necessary to test reticulocytes and immature white blood cells in blood samples. [0003] US Patent No. 5,492,833 discloses a method for removing hemoglobin in erythrocytes b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 张华利刘林陈庚文张子千余珊
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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