Method for simultaneously detecting two glucocorticoid isomerides in animal-derived food

Abstract

The invention discloses a method for simultaneously detecting two glucocorticoid isomerides in animal-derived food. The method separates two enantiomers of betamethasone, dexamethasone, fluorometholone and desoximetasone by adopting a conventional chromatographic column, and can detect the residual amount of betamethasone, dexamethasone, fluorometholone and desoximetasone in the animal-derived food and confirm. A C18 chromatic column is adopted for liquid chromatogram; the flow phases are respectively acetonitrile containing methyl alcohol and water containing methyl alcohol, and tandem mass spectrum detection is carried out; two enantiomers of betamethasone, dexamethasone, fluorometholone and desoximetasone can be subjected to chromatograph separation; the detection limit of dexamethasone is 0.2 micron g / kg; the detection limit of betamethasone, fluorometholone and desoximetasone is 0.4 micron g / kg; the residual amount regulatory restriction requirement at home and abroad is met; the method is simple, convenient, accurate, flexible and reliable.

Description

Technical field [0001] The invention belongs to the field of analytical chemistry and food safety detection, and relates to a liquid chromatography-tandem mass spectrometry detection method for betamethasone, dexamethasone, fluorometholone and deoxymethasone in animal source food, especially for the detection of betamethasone Chromatographic separation and confirmatory analysis of two pairs of isomers with dexamethasone, fluorometholone and deoxymethasone. Background technique [0002] Betamethasone, Dexamethasone, Fluorometholone and Desoxinetasone belong to glucocorticoid veterinary drugs, which are abused and misused in the breeding industry, making some animal sources Residues of such hormonal veterinary drugs will appear in food. Long-term consumption of animal-sourced foods containing glucocorticoid veterinary drugs will affect the hormone balance of the body, which may cause abnormal development in young children and may cause cancer. The maximum residues of glucocor...

AI Technical Summary

Problems solved by technology

However, since betamethasone and dexamethasone, fluorometholone and deoxymethasone are two pairs of epimers, the diastereoisomers that differ only in the configuration of carbon atoms are very similar in chromatographic behavior, Difficult to separate completely

Usually the separation of such isomers requires the use of unconventional chiral chromatographic separation columns, but the cost is high and it is difficult to be widely used

None of the current detection and analysis methods can use conventional chromatographic columns to chromatographically separate the two pairs of isomers, so that when the sample is positive, it is impossible to judge whether it contains a mixture or a single isomer, and it is impossible to confirm which isomer it is , which brings difficulties to the judgment of the test results and easily leads to misjudgment

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