Preparation method for cross-linked sodium hyaluronate gel with asymmetric structure

A technology of cross-linking hyaluronic acid and sodium hyaluronate, which is applied in medical biomaterials and the medical field, can solve the problems of low utilization rate of cross-linking agent and difficulty in removing cross-linking agent, achieve long residual time in vivo, improve enzyme resistance The effect of reducing capacity and improving utilization

Active Publication Date: 2015-07-08
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Usually the preparation of cross-linked sodium hyaluronate gel is carried out with a fixed concentration of reactants. In such a reaction, the utilization rate of the cross-linking agent is low. In order to achieve a better cross-linking effect, more cross-linking agents are usually added. Reaction of the cross-linking agent, so that the removal of the cross-linking agent is more difficult

Method used

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  • Preparation method for cross-linked sodium hyaluronate gel with asymmetric structure

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Experimental program
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Effect test

Embodiment 1

[0021] Prepare 20ml of 1% sodium hydroxide aqueous solution, add 0.02g of cross-linking agent BDDE, mix well, add 2.0g sodium hyaluronate, stir well, put it in a 10°C reaction box, set the dehydration rate to 3% / day , after reacting for 10 days, put it into a 40°C water bath, heat for 2 hours, take out the gel cut into about 0.5g in mass, put it in PBS buffer solution, and perform swelling dialysis to obtain cross-linked sodium hyaluronate gel. 20mg / ml.

[0022]

Embodiment 2

[0024] Prepare 20ml of 1% sodium hydroxide aqueous solution, add 0.01g of cross-linking agent BDDE, mix well, add 2.0g of sodium hyaluronate, stir well, put it in a 10°C reaction box, set the dehydration rate to 4% / day , after reacting for 10 days, put it into a 40°C water bath, heat for 2 hours, take out the gel cut into about 0.5g in mass, put it in PBS buffer solution, and perform swelling dialysis to obtain cross-linked sodium hyaluronate gel. 20mg / ml.

[0025]

Embodiment 3

[0027] Prepare 20ml of 1.5% sodium hydroxide aqueous solution, add 0.007g of cross-linking agent BDDE, mix well, add 2.0g of sodium hyaluronate, stir well, put it in a 10°C reaction box, set the dehydration rate to 5% / day , after reacting for 10 days, put it into a 40°C water bath, heat for 2 hours, take out the gel cut into about 0.5g in mass, put it in PBS buffer solution, and perform swelling dialysis to obtain cross-linked sodium hyaluronate gel. 20mg / ml.

[0028]

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Abstract

The invention relates to a preparation method for a cross-linked sodium hyaluronate gel with an asymmetric structure. The method comprises the steps: adopting a sodium hyaluronate solution containing a cross-linking agent as a starting material, adding a precious small amount of the cross-linking agent, under conditions of constant temperature and constant humidity, carrying out gradient concentration dehydration, then dialyzing the cross-linked sodium hyaluronate gel in a buffer solution, dialyzing until the gel is no longer expanded, and finally, granulating to obtain the cross-linked sodium hyaluronate gel with the asymmetric structure. The cross-linked sodium hyaluronate obtained by the preparation method provided by the invention has the gradient asymmetric structure in microcosmic scale and shows the characteristics of dense exterior and loose interior; the structure ensures that the cross-linked sodium hyaluronate gel can effectively buffer attacking action of sodium hyaluronate enzymes, the survival time in bodies is greatly extended, and at the same time, excellent biological safety is also showed with precious small addition amount of the cross-linking agent.

Description

Technical field [0001] The invention involves the technology field of medical biomagomy, which is a preparation method involving a new type of sodium hyaluronate. The sodium hyaluronate is used in medical filling beauty and belongs to the medical field. Background technique [0002] Sodium hyaluronate is an important component of human and animal skin, vitreous, joint lubrication and cartilage tissue. It is (1 -β -4) D -Glytic acid and (1 -β -3) N -Acetyl- D -The amino glucose dual -sugar units are repeatedly connected, widely used in repairing surgery, eye surgery, or as beauty filling materials.Sodium hyaluronate has good physical and chemical performance and biocompatibility, but it is rapidly degraded by the role of specific hyaluronase in the body. The half-life is only 2-3 days.EssenceSodium hyaluronate is a polymer gel obtained by sodium hyaluronate through chemical cross -linked modification, which makes up for the shortcomings of short savings of sodium hyaluronate, and ...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L5/08C08K5/41C08K5/1515A61L27/20A61L27/52
Inventor 魏长征奚宏伟蒋丽霞
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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