Mesorhizobium zengchengense and application thereof

A technology of slow rhizobium and inoculum, which is applied in the field of microorganisms to achieve broad application prospects and strong resistance to stress.

Inactive Publication Date: 2015-07-08
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the bottleneck restricting the development of my country'

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  • Mesorhizobium zengchengense and application thereof
  • Mesorhizobium zengchengense and application thereof
  • Mesorhizobium zengchengense and application thereof

Examples

Experimental program
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Embodiment 1

[0018] Example 1: Isolation and Identification of Bradyrhizobium RITF1218, RITF907, RITF908 in Zengcheng

[0019] 1. Isolation of Rhizobium

[0020] The strains were isolated from root nodules of Acacia hemanthus in nurseries in Huizhou City and Zengcheng City, Guangdong Province. Rinse the surface of the nodules with tap water to clean the sediment, soak them in 95% alcohol for 30 seconds, put them into 0.1% mercuric chloride solution for surface disinfection for 5 minutes, and rinse them with sterile water for 8 times. Put the root nodules after surface disinfection between two sterile glass slides, squeeze them hard to break the root nodules, use a sterile inoculation needle to dip the root nodule liquid, draw a line on the YMA plate, and culture in a constant temperature incubator at 28-30°C When colonies appear, typical colonies are picked for purification and identification. Thus, rhizobia RITF1218, RITF907, and RITF908 were purified.

[0021] 2. Identification of Rhi...

Embodiment 2

[0047] Example 2. Effects of Mesorhizobium zengchengense RITF1218 inoculated with Acacia blackwood, Acacia spp., Jacaranda and Leucaena in Zengcheng.

[0048] 1. Bacteria culture

[0049] Mesorhizobium zengchengense (Mesorhizobium zengchengense) RITF1218 was activated on the slant and then transferred to YMA liquid medium, and cultured on a shaking table at 28-30°C (150 rpm) to the late logarithmic growth stage to obtain Zengcheng mesorhizobium nodules Bacteria (Mesorhizobium zengchengense) RITF1218 bacterial liquid.

[0050] 2. Seed treatment and germination

[0051] First soak the seeds with concentrated sulfuric acid for half an hour, then pour out the concentrated sulfuric acid, wash with sterile water for 7 to 8 times, and soak in sterile water until the seeds swell. The treated seeds were placed in a sterile petri dish (inner lined with sterile filter paper and sterile water for moisturizing), and placed in a 30°C incubator to germinate.

[0052] 3. Inoculation

[00...

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Abstract

The invention discloses Mesorhizobium zengchengense and application thereof. The Mesorhizobium zengchengense RITF1218 is preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, China, on Oct. 17th, 2014, with an accession number of CCTCC NO: M2014492. The Mesorhizobium zengchengense RITF1218 is separated and screened from root nodules of Acacia melanoxylon, can bond with Acacia melanoxylon, Acacia aneura, Albizia falcataria, Leucaena leucocephala and the like, has strong stress resistance, and has wide application prospects in production of nitrogen-fixing microbial agents and bio-organic fertilizers.

Description

Technical field: [0001] The invention belongs to the field of microorganisms, and in particular relates to a Zengcheng mesorhizobium and its application. Background technique: [0002] Biological nitrogen fixation refers to the process in which some bacteria, etc., can convert molecular nitrogen in the atmosphere into ammonia nitrogen that plants can directly absorb and utilize. 78% of nitrogen in the atmosphere can only be utilized through biological nitrogen fixation. Nitrogen is one of the elements necessary for the growth and development of animals, plants and microorganisms. In order to improve economic benefits, the chemical industry has been vigorously developed in the past to produce chemical fertilizers to improve soil fertility and promote the rapid growth of plants. However, the extensive use of chemical fertilizers consumes energy, pollutes water and soil, and even destroys the ecological balance. The world's population, resources, and environment have become g...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 陆俊锟朱亚杰康丽华
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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