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A kind of aqueous adjuvant and application of porcine circovirus type 2 inactivated vaccine

A porcine circovirus and water-based adjuvant technology, which is applied in the field of swine vaccines to achieve the effects of high specific antibody, simple process and fast specific antibody

Active Publication Date: 2018-02-13
WUHAN KEQIAN BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Highly hydrophilic proteins cannot be incorporated into ISCOM

Method used

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  • A kind of aqueous adjuvant and application of porcine circovirus type 2 inactivated vaccine
  • A kind of aqueous adjuvant and application of porcine circovirus type 2 inactivated vaccine
  • A kind of aqueous adjuvant and application of porcine circovirus type 2 inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Utilizing the adjuvant composition of the present invention to prepare porcine circovirus type 2 inactivated vaccine (WH strain)

[0066] Carry out proportioning according to different composition content in the adjuvant composition, carry out emulsification respectively with 65ml porcine circovirus type 2 virus liquid, prepare 100ml porcine circovirus type 2 inactivated vaccines (see table 1), the vaccine name of making Group 1, Group 2, Group 3, Group 4 are used in the following examples.

[0067] Table 1: Components of vaccines prepared with different adjuvant formulations (volume ml)

[0068]

[0069] The specific operation method is as follows:

[0070] a) Using PH7.2 PBS buffer solution to adjust the virus content of the inactivated virus solution of porcine circovirus type 2-WH strain (CCTCC NO: V201333) to obtain the virus stock solution;

[0071] b) Add Carbopol 974p to the composition of step a and mix well;

[0072] c) Add Montanide GEL 01 to the compos...

Embodiment 2

[0079] Embodiment 2: vaccine stability comparative test

[0080] 4 groups of porcine circovirus type 2 inactivated vaccines prepared by embodiment 1 and the porcine circovirus type 2 inactivated vaccine stability contrast tests prepared by 201 adjuvant and Marcol52 adjuvant

[0081] The 4 groups of porcine circovirus type 2 inactivated vaccines prepared in Example 1 and the porcine circovirus type 2 inactivated vaccines prepared with 201 adjuvant and Marcol52 adjuvant were placed at 37°C, 25°C, and 4°C for observation respectively. The stability of the vaccine was regularly sampled and observed (test results are shown in Table 2, Table 3, and Table 4).

[0082] The test results show that the porcine circovirus type 2 inactivated vaccine prepared with the adjuvant developed by this invention has good stability, especially the first group of adjuvants, which are the best at 37°C, 25°C, and 4°C .

[0083] Table 2 Observation of Stability Days at 37°C

[0084]

[0085] Table...

Embodiment 3

[0094] Experiment on the law of growth and decline of vaccine antibodies prepared by different adjuvant compositions

[0095] 4 groups of porcine circovirus type 2 inactivated vaccines prepared in Example 1 and the porcine circovirus type 2 inactivated vaccines prepared by 201 adjuvant and Marcol52 adjuvant comparative test on immune growth and decline laws

[0096] 1. After detecting the main pathogens and related antibodies on a pig farm, select 21-25 day-old pigs with negative pathogens such as porcine circovirus type 2, swine fever virus, porcine reproductive and respiratory syndrome virus, and porcine parvovirus. Circovirus type 2 antibody-negative pigs.

[0097] 2. 35 antibody-negative pigs will be repurchased, randomly divided into 7 groups, 5 pigs in each group, and 1 group to 6 groups will be inoculated with 1 vaccine (10 pigs) through the neck muscle. 7 TCID50 / head portion). Group 7 (during the feeding process, one of them became ill. Finally, this pig was eliminat...

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Abstract

The invention discloses a water-based adjuvant of porcine circovirus type 2 inactivated vaccine and its application. The adjuvant composition of the invention can significantly improve the immune effect of porcine circovirus type 2 inactivated vaccine, and can stimulate the body to produce growth Up to 150 days of high-efficiency protection. At the same time, the preparation process of the vaccine is stable, and the side effects caused by the vaccine immunization are small and have unique advantages. The preparation process of the adjuvant composition is simple, does not require large-scale equipment, has low cost, and is convenient for large-scale production of vaccines.

Description

technical field [0001] The invention mainly relates to the field of pig vaccines. Specifically relates to an aqueous adjuvant and application of porcine circovirus type 2 inactivated vaccine. Background technique [0002] Porcine circovirus type 2 (PCV2) is the main pathogen of weaned piglet multisystem wasting syndrome (PMWS), which is characterized by immunosuppression and multisystem failure in weaned piglets. The disease mainly occurs in weaned piglets aged 6-15 weeks. The sick pigs mainly show clinical symptoms such as poor growth and development, dyspnea, anemia, diarrhea, weakness, enlarged lymph nodes, slow weight gain, and jaundice, and can also cause infection. The immune function of piglets is damaged, leading to a decline in the body's immunity, which leads to secondary infections and aggravates the occurrence of the disease. The main pathological features are the loss of lymphocytes in lymphoid tissue and the infiltration of inflammatory granulocytes. In addi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/12A61P31/20
Inventor 陈波周明光徐高原方雨林郝根喜曾爱星陈章表金建云
Owner WUHAN KEQIAN BIOLOGY CO LTD
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