Glycan composition for activating immunity to treat pathogen infection
A technology of composition and polysaccharide, which is applied in the direction of antibacterial, antifungal, antiviral, etc., and can solve the problems of inability to treat viral diseases, unstable effect, and reproductive barriers of breeding pigs.
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Embodiment approach 1
[0023] Add 10g of chitosan to 500ml of 0.5 mole hydrochloric acid, add 5g of octanoic acid, stir well at 35°C, add 15g of aluminum sulfate, 2g of calcium dextran, 20g of dipotassium hydrogen phosphate, and 10g of zinc threonine Stir well and evenly at 25°C. The pH value was adjusted to 2.9 with sulfuric acid to prepare a polysaccharide composition containing functional elements.
Embodiment approach 2
[0025] Using the classic in vitro anti-hepatitis B virus drug screening model HepG2.2.15 cell model, the composition of different concentrations was administered to HepG2.2.15 cells for several days, the cell supernatant was taken, and the hepatitis B surface antigen (HBsAg) and e antigen (HBeAg) were measured. And HBVDNA secretion to judge the drug effect. During the experiment, the cytotoxicity of the composition was determined by MTT method. The antiviral drug lamivudine (3TC), which has been proven effective in clinical application, was selected as the control.
[0026] When the HepG2.2.15 cells proliferate to a certain extent and the antigen secretion is stable, count the cells, take the cell suspension and culture it in a 96-well culture plate, 100 μL per well, so that the concentration of cells per well reaches 2000, after 24 hours of culture, aspirate The cell supernatant was added with different concentrations of the composition to continue culturing for 6 days, duri...
Embodiment approach 3
[0038] Select 30 pigs with symptoms of virus infection: progressive emaciation or growth retardation, anorexia, depression, slow movement, pale skin, disheveled coat, dyspnea, cough, etc. Divided into a treatment group and a control group, the age, clinical manifestations and disease severity of pigs in the two groups were basically the same. There are 15 cases in the treatment group and the control group. In the treatment group, 10ml of the composition is added to 500ml of drinking water, and each pig is supplied with 500ml / day. The control group was kept in isolation and managed routinely. After the treatment, the mental state, body temperature and feed intake of the pigs were observed every day. After 5 days of medication, the clinical symptoms of sick pigs disappeared, and those with normal body temperature, appetite, and mental state were judged as cured; after 5 days of medication, the sick pigs’ appetite and mental state improved, and those with normal body temperature...
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