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Chimpanzee adenovirus vaccine for preventing and treating highly-expressed WT1 tumor

An adenovirus and chimpanzee technology, applied in the field of vaccine creation, can solve problems such as unsatisfactory clinical effects

Inactive Publication Date: 2015-07-22
INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among the multiple clinical trials of peptide vaccines against WT1 approved by the National Institute of Health (NIH), some can produce moderate antigen-specific immune responses and certain protective effects, but their clinical effects are not satisfactory

Method used

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  • Chimpanzee adenovirus vaccine for preventing and treating highly-expressed WT1 tumor
  • Chimpanzee adenovirus vaccine for preventing and treating highly-expressed WT1 tumor
  • Chimpanzee adenovirus vaccine for preventing and treating highly-expressed WT1 tumor

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Embodiment Construction

[0036] The invention will be further described below through specific embodiments in conjunction with the accompanying drawings.

[0037] 1. Virus Synthesis and Amplification

[0038] 1.1 Synthesis of tWT1 in pUC57-Amp plasmid

[0039] The tWT1 inpUC57-Amp plasmid was synthesized by Jinweizhi Biotechnology (Beijing) Co., Ltd. Plasmid map and sequence see figure 1 . The enzyme cutting sites are Xba I and Kpn I.

[0040] 1.2 (GM-CSF)-P2A-(IL-4)-F2A-(WT1)in pUC57-Amp plasmid synthesis

[0041](GM-CSF)-P2A-(IL-4)-F2A-(WT1)in pUC57-Amp plasmid was synthesized by Jinweizhi Biotechnology (Beijing) Co., Ltd. Plasmid map and sequence see figure 2 . The enzyme cutting sites are Xba I and Kpn I.

[0042] 1.3 Construction of recombinant adenovirus vector pAdC68-WT1

[0043] The tWT1 in pUC57-Amp plasmid was digested with Xba I and Kpn I, and cloned into the Xba I and Kpn I sites of pShuttle to form pShuttle-WT1. Digest pShuttle-WT1 with I-CeuI and PI-SceI, and clone the excised...

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Abstract

The invention discloses a chimpanzee adenovirus vaccine for preventing and treating highly-expressed WT1 tumor. A new means for treating breast cancer is provided, a WT1 (Wilm's tumor 1) gene is selected as a target site, a chimpanzee adenovirus is selected as a vector, and a recombinant chimpanzee adenovirus vaccine for expressing WT1 and (GM-CSF)-P2A-(IL-4)-F2A-(WT1) is used for successfully preventing breast cancer and prolonging the lifetime of mice suffering from breast cancer. An experimental foundation is laid for the clinical application of the vaccine, and a prompting effect for the treatment on other tumors is achieved.

Description

technical field [0001] The invention belongs to the technical field of vaccines, and in particular relates to a chimpanzee adenovirus vaccine for preventing and treating tumors highly expressing WT1. Background technique [0002] Breast cancer is one of the common breast tumors, and its incidence rate ranks first among female cancers. At present, the curative effect of traditional treatment methods based on radiotherapy, chemotherapy and surgery is not satisfactory, especially for highly metastatic breast cancer, so it is urgent to develop new treatment methods. [0003] The WT1 gene is located at 11p13, consists of 10 exons, and encodes a transcription factor with a zinc finger structure. It is the earliest tumor suppressor gene found to be related to the occurrence and development of Wilm tumors. WT1 is abnormally expressed in a variety of tumors, but due to different cell characteristics, it can function as an oncogene or a tumor suppressor gene. WT1 is highly expressed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K39/235A61P35/00
Inventor 冯晓明周东明邢曼吴万通罗悦晨方春敏
Owner INST OF HEMATOLOGY & BLOOD DISEASES HOSPITAL CHINESE ACADEMY OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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