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Sample diluting solution for ELISA, and preparation method thereof

A sample diluent and liquid technology, applied in the field of ELISA sample diluent and its preparation, can solve the problems affecting the accuracy and repeatability of the test results, obvious matrix effect, and inability to effectively protect the sample to be tested, so as to improve the accuracy Sexuality and repeatability, reduced noise, dramatic results

Active Publication Date: 2015-08-05
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage is that the production cost is low, and the disadvantage is that only salt buffer solution can not effectively protect the sample to be tested, and it is quite different from the serum environment, and the matrix effect is obvious, which affects the accuracy and repeatability of the test results

Method used

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Examples

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preparation example Construction

[0016] The preparation method of the sample diluent for ELISA comprises the following steps: taking the preparation of 1 liter of sample diluent for ELISA as an example, it includes the following steps:

[0017] Step 1. Weigh 0.386g of sodium dihydrogen phosphate dihydrate, 1.93g of disodium hydrogen phosphate dodecahydrate and 8.2g of sodium chloride in a container, measure 1000ml of purified water into the container, stir until completely dissolved, and obtain salt buffer;

[0018] Step 2. Weigh 15g of serum protein and add it into the salt buffer solution, and continue to stir for 3-5 minutes to obtain solution A;

[0019] Step 3: Add 1.0g of adenosine diphosphate, 3.0g of ethylenediaminetetraacetic acid and 2ml of liquid preservative to solution A, stir until completely dissolved, and finally add purified water to make the volume to 1L, stir well and use it for ELISA Sample diluent.

[0020] The liquid preservative is ProClin300.

[0021] The serum protein is bovine ser...

Embodiment

[0042] A sample diluent for ELISA is composed of serum protein, adenosine diphosphate, ethylenediaminetetraacetic acid, saline buffer solution, liquid preservative and purified water, and the mass percentage of each component is: 1.5% serum albumin, 0.1% adenosine diphosphate, 0.3% ethylenediaminetetraacetic acid, 0.1% saline buffer and 0.2% liquid preservative, the balance is purified water;

[0043] The salt buffer contains 0.386g sodium dihydrogen phosphate dihydrate, 1.93g disodium hydrogen phosphate dodecahydrate and 8.2g sodium chloride in every liter of salt buffer, and the balance is purified water.

[0044] The preparation method of the sample diluent for ELISA comprises the following steps: taking the preparation of 1 liter of sample diluent for ELISA as an example, it includes the following steps:

[0045] Step 1. Weigh 0.386g of sodium dihydrogen phosphate dihydrate, 1.93g of disodium hydrogen phosphate dodecahydrate and 8.2g of sodium chloride in a container, meas...

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Abstract

A sample diluting solution for ELISA is composed of a serum protein, adenosine diphoshate, ethylene diamine tetraacetic acid, a salt buffering solution, a liquid antiseptic and purified water. A preparation method of the sample diluting solution comprises the following steps: weighing sodium dihydrogen phosphate dehydrate, disodium hydrogen phosphate dodecahydrate and sodium chloride, placing above weighed components in a container, measuring purified water, adding purified water into the container, and stirring until complete dissolving to obtain the salt buffering solution; weighing the serum protein, and adding the serum protein into the salt buffering solution; and adding adenosine diphoshate, ethylene diamine tetraacetic acid and the liquid antiseptic, stirring until complete dissolving, and adding purified water to a constant volume in order to obtain the sample diluting solution for ELISA. Compared with sample diluting solutions generally used in the industry at present, the sample diluting solution for ELISA disclosed in the invention has the advantages of sample protection, effective reduction of the interference of matrix effect, and improvement of the accuracy and repeatability of a detection result; and relevant experiments verify that the sample diluting solution for ELISA has a substantial effect.

Description

technical field [0001] The invention relates to a sample diluent, in particular to a sample diluent for ELISA and a preparation method thereof. Background technique [0002] At present, the sample diluent used in the industry is basically PBST solution, which is composed of disodium hydrogen phosphate dodecahydrate, potassium dihydrogen phosphate, sodium chloride, potassium chloride, and Tween 20. The advantage is that the production cost is low, and the disadvantage is that only salt buffer solution can not effectively protect the sample to be tested, and it is quite different from the serum environment, and the matrix effect is obvious, which affects the accuracy and repeatability of the test results. Contents of the invention [0003] The purpose of the present invention is to solve the deficiencies of the above-mentioned technical problems, to provide a sample diluent for ELISA, use the diluent to dilute the ELISA sample, reduce the interference of the matrix effect, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38
Inventor 常慧云邵军军林彤赵付荣张永光王善普李秀梅孟晓梅赵韵祺
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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