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Method for increasing diosgenin content in Smilax root tuber through foliage spraying of bacteriocin

A technology of diosgenin and foliar spraying, which is applied in fertilization methods, gardening methods, botanical equipment and methods, etc., can solve the problems of failing to meet the requirements of the Pharmacopoeia, having no medicinal value of sarsaparilla, and having a great influence on the place of production , to achieve the effect of improving medicinal value, broad market application prospects and significant benefits

Inactive Publication Date: 2015-08-12
JISHOU UNIVERSITY
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  • Summary
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The content of diosgenin in smilax root tubers is greatly affected by the place of origin. The content of diosgenin in smilax tuber roots produced in some areas exceeds the pharmacopoeia standard by hundreds of times. Sarsaparilla produced in the region has little medicinal value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Preparation of Fusarium oxysporum: Inoculate Fusarium oxysporum into the seed culture solution, cultivate for 50 hours at 29°C with a rotational speed of 150rpm to obtain the seed solution; Fermentation culture broth, fermentation temperature 30°C, rotation speed 125 rpm, fermentation culture time 4.1d; after the fermentation culture broth was centrifuged and filtered, the mycelium of Fusarium oxysporum was obtained, and the mycelium was extracted with methanol or ethanol solution to obtain pointed Fusarium sporogenes.

[0016] (2) For the spraying of Fusarium oxysporum, the above-mentioned Fusarium oxysporum was formulated into a 0.08% aqueous solution, and the prepared solution was sprayed on the leaves of growing Smilax 1.1 months before and after the flowering period, Spray before sunrise or after sunset, spray once a day, and the dosage of each spray is to make the leaves evenly stained with the solution.

[0017] After foliar spraying by this method, the dios...

Embodiment 2

[0019] (1) Preparation of Fusarium oxysporum: Inoculate Fusarium oxysporum into the seed culture solution, cultivate the seed solution for 60 hours at 34°C and 130 rpm; inoculate the seed solution with an inoculation volume of 8% In the expanded fermentation culture medium, the fermentation temperature is 30°C, the rotation speed is 140rpm, and the fermentation culture time is 5 days; after the fermentation culture medium is centrifuged and filtered, the mycelium of Fusarium oxysporum is obtained, and the mycelium is extracted with methanol or ethanol solution to obtain sharp Fusarium sporogenes;

[0020] (2) For the spraying of Fusarium oxysporum, the above-mentioned Fusarium oxysporum is prepared into a 0.09% aqueous solution, and the prepared solution is sprayed on the leaves of growing Smilax 1.5 months before and after the flowering period, Spray before sunrise or after sunset, spray once every 2 days, and the dosage of each spray is to make the leaves evenly stained with...

Embodiment 3

[0024] (1) Preparation of Fusarium oxysporum: Inoculate Fusarium oxysporum into the seed culture solution, cultivate the seed solution for 70 hours at 31°C and 140 rpm; Expand the fermentation culture medium, the fermentation temperature is 31°C, the rotation speed is 155 rpm, and the fermentation culture time is 4.5 days; after the fermentation culture medium is centrifuged and filtered, the mycelia of Fusarium oxysporum are obtained, and the mycelium is extracted with methanol or ethanol solution. Fusarium oxysporum;

[0025] (2) For the spraying of Fusarium oxysporum, prepare the above-mentioned Fusarium oxysporum into a 0.06% aqueous solution, and spray the prepared solution on the leaves of growing Smilax 2 months before and after the flowering period, Spray before sunrise or after sunset, spray once every 3 days, and the dosage of each spray is to make the leaves evenly stained with the solution.

[0026] The preferred preparation method of the expanded fermentation bro...

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Abstract

The invention discloses a method for increasing diosgenin content in a Smilax root tuber through foliage spraying of bacteriocin. The method comprises the following steps: (1) preparation of fusarium oxysporum bacteriocin: a step of subjecting fusarium oxysporum to inoculation and enlarge culture so as to obtain mycelium and extracting fusarium oxysporum bacteriocin from the obtained mycelium by using a methanol or ethanol solution; and (2) spraying of fusarium oxysporum bacteriocin: a step of preparing an aqueous fusarium oxysporum bacteriocin solution with a concentration of 0.05% to 0.1% and carrying out foliar spraying once every one to three days before sunrise or after sunset in 1 to 2 months before or after a flowering period, wherein the spraying amount of the solution every time allows leaf surfaces to be uniformly stained with the aqueous solution. Through the foliar spraying method in the invention, the diosgenin content of a Smilax root tuber dug in winter of the year is 10% to 20% higher than the diosgenin content of a Smilax root tuber dug under same conditions without foliar spraying, and increase of the diosgenin content is very significant; meanwhile, industrialization, scalization and standardization implementation of the method is easy, and the method has broad application prospects.

Description

technical field [0001] The invention relates to a method for increasing the content of diosgenin in sarsaparilla tuber roots by spraying mycosin on leaves, specifically referring to spraying Fusarium oxysporum on leaves before and after the flowering stage to increase the diosgenin content in sarsaparilla tuber roots method. Background technique [0002] Smilax is a perennial deciduous climbing shrub of the genus Smilax in the family Liliaceae. It was officially included in the "Chinese Pharmacopoeia" in 2010. A large amount, boils and carbuncles. Sarsaparilla is mainly used as medicine from the root tubers that grow underground. The traditional Chinese medicine preparations developed from the raw materials of Smilax are used to treat chronic pelvic inflammatory disease and adnexitis. The curative effect is remarkable, and the market demand is relatively large. [0003] The most important medicinal ingredient in Smilax is diosgenin, and the "Chinese Pharmacopoeia" stipulat...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01C21/00
CPCA01G7/06A01C21/005
Inventor 贺建武陈功锡田启建陈义光刘祝祥田向荣魏华李贵
Owner JISHOU UNIVERSITY
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