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A method for extracting high-content natural vitamin E from annatto

A technology of natural vitamins and annatto, applied in the direction of organic chemistry, can solve the problems of complicated process and unsuitable for large-scale industrial production, and achieve the effect of reducing cost, easy operation and reducing damage

Active Publication Date: 2017-05-17
SHAANXI YUANBANG BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are very few documents on the extraction of natural VE from annatto, and the disclosed process is extremely complicated. For example, in US20130178519A1, it takes more than ten steps to obtain the final powder product, and the product content is only 15.8%, which is not suitable for large-scale production. scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A: Extraction: 140g of annatto was extracted with 700mL of anhydrous methanol at 20°C with stirring for 2 hours, suction filtered, the filter cake was washed with 300mL of anhydrous methanol, and the filtrate was collected. B: Ionic resin adsorption: The filtrate from the previous step is adsorbed by 200mL ionic resin. After the adsorption is completed, it is washed with 400mL of anhydrous methanol. C: Reverse resin adsorption: Adsorb the analytical solution in the previous step with 100 mL of reverse resin, analyze with 500 mL of anhydrous methanol after the adsorption, and concentrate and dry the analytical solution to obtain 12.5 g of crude product. D: Decolorization: Dissolve the product from the previous step with 60 mL of petroleum ether, add 1.2 g of activated clay and 1.2 g of activated carbon, stir at 20° C. for 1 h, filter with suction, and use the filtrate for later use. E: Silica gel column chromatography: the filtrate from the previous step is adsorbed by a...

Embodiment 2

[0039] A: Extraction: 140g of annatto was extracted with 700mL of absolute ethanol with stirring at 20°C for 2h, suction filtered, the filter cake was washed with 300mL of absolute ethanol, and the filtrate was collected. B: Ionic resin adsorption: The filtrate from the previous step is adsorbed by 200mL ionic resin, washed with 400mL of absolute ethanol after the adsorption is completed, and analyzed with 1000mL of 0.2% hydrochloric acid ethanol solution after washing, and the analytical solution is set aside. C: Reverse resin adsorption: Adsorb the analytical solution in the previous step with 100 mL of reverse resin, analyze with 500 mL of absolute ethanol after the adsorption, and concentrate and dry the analytical solution to obtain 13.0 g of crude product. D: Decolorization: Dissolve the product from the previous step with 60 mL of petroleum ether, add 1.3 g of activated clay and 1.3 g of activated carbon, stir at 20° C. for 1 h, filter with suction, and use the filtrate ...

Embodiment 3

[0041] A: Extraction: 140g of annatto was extracted with 700mL of absolute ethanol at 20°C with stirring for 1 hour, suction filtered, the filter cake was washed with 300mL of 95% ethanol, and the filtrate was collected. B: Ionic resin adsorption: The filtrate from the previous step is adsorbed by 200mL ionic resin, washed with 400mL 95% ethanol after the adsorption is completed, analyzed with 1000mL 0.2% hydrochloric acid ethanol solution after washing, and the analytical solution is set aside. C: Reverse resin adsorption: Adsorb the analysis solution in the previous step with 100 mL reverse resin, analyze with 500 mL 95% ethanol after the adsorption, concentrate and dry the analysis solution to obtain 11.5 g of crude product. D: Decolorization: Dissolve the product from the previous step with 60 mL of petroleum ether, add 1.2 g of activated clay and 1.2 g of activated carbon, stir at 20° C. for 1 h, filter with suction, and use the filtrate for later use. E: Silica gel colum...

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Abstract

The present invention provides a method for extracting high content natural vitamin E from annatto. The method is simple in operation and high in yield, and can obtain natural vitamin E with content more than 90%. The purification process uses 8%-9% annatto as the raw material and includes the following steps: (1) extracting annatto with an organic solvent at 20-40 DEG C for 1-3 h, filtering off the insolubles, and collecting the filtrate; (2) adsorbing the filtrate by ion resin; washing out the impurities with a solvent after adsorption, and analyzing to obtain an analytical solution for standby; (3) conducting reverse resin adsorption on the analytical solution, analyzing, concentrating to dry the analytical solution, so as to obtain a crude product; (4) dissolving the crude product with petroleum ether, adding activated clay and activated carbon, stirring at 20-40 DEG C, and carrying out vacuum filtration to obtain a filtrate for standby; and (5) adsorbing the filtrate with a silica gel column, eluting by a mixed solvent containing petroleum ether and ethyl acetate, and concentrating to dry the eluent to obtain a final product of light yellow oil.

Description

technical field [0001] The invention relates to a method for extracting high-content vitamin E from annatto. Background technique [0002] Natural vitamin E (VE), also known as tocopherol, includes 8 kinds of substances such as α, β, δ, γ type tocopherol and corresponding tocotrienol, and has unique physiological functions, such as anti-free radical, anti-aging, Prevent skin keratinization, improve the physical immune function and cellular immune function of human and animals, prevent and treat cardiovascular and cerebrovascular diseases, maintain and enhance reproductive function and many other important physiological functions. VE has two sources: natural VE and synthetic VE. Natural VE is mixed tocopherols and tocotrienols containing various monomers. Natural VE is superior to synthetic VE in terms of physiological activity and antioxidant capacity, and natural VE has The human body has no toxic and side effects, so natural VE is favored by consumers in industries such a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/72
CPCC07D311/72
Inventor 祝木林张永利寇玉锋
Owner SHAANXI YUANBANG BIO TECH
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