Application of platelet-poor plasma in skin care
A platelet-poor plasma and skin care technology, applied in the application field of platelet-poor plasma in skin care, can solve problems such as short effect time, achieve good nursing effect, accelerate cell proliferation, and promote healing.
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[0025] The preparation method of PPP
[0026] 1) Centrifuge the blood sample once, centrifuge at 1400-1600rpm for 8-12min, take out the platelet-rich plasma (PRP) in the upper layer, and obtain PRP once;
[0027] 2) Centrifuge the above primary centrifuged PRP for a second time at 2800-3200rpm for 18-22min to concentrate the platelets so that the final concentration of platelets reaches 800×10 9 ~1200×10 9 per L; the supernatant obtained after centrifugation is PPP (platelet-poor plasma).
Embodiment 1
[0029] The preparation method of embodiment 1 PPP
[0030] The specific steps of obtaining PPP at the same time when preparing PRP are as follows:
[0031] 1) Whole blood routine test (platelet count);
[0032] 2) Perform a centrifugation according to the specific situation of the patient (generally, women use 1500rpm, 10min), and use a sterile syringe to separate the upper layer of platelet-rich plasma (PRP) to obtain a centrifuged PRP;
[0033] 3) Take 50 μl (microliter) of the primary centrifuged PRP obtained in the previous step for platelet count;
[0034] 4) According to the counting results, the above-mentioned primary centrifugal PRP was divided into two parts, the first part was reserved for the last; the second part was subjected to secondary centrifugation (3000rpm, 20min) to concentrate platelets, so that the final concentration of platelets reached 1000×10 9 / L; the supernatant obtained after centrifugation is PPP (platelet-poor plasma), take it out and transfer...
Embodiment 2
[0035] The preparation method of embodiment 2 PPP
[0036] 1) Centrifuge the blood sample once at 1500rpm for 10min, and use a sterile syringe to separate the upper layer of platelet-rich plasma (PRP) to obtain PRP once;
[0037] 2) Centrifuge the above primary centrifuged PRP for a second time at 3000rpm for 20min to concentrate platelets so that the final concentration of platelets reaches 1000×10 9 per L; the supernatant obtained after centrifugation is PPP (platelet-poor plasma).
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