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Water gel material with thrombin responsive thrombolysis capability and preparation method thereof

A technology of hydrogel and thrombin, applied in the field of biomedical polymer materials and polymer chemistry, can solve the problem of self-acceleration of blood coagulation reaction

Active Publication Date: 2015-08-26
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, thrombin will also feedback and activate coagulation factors upstream of the coagulation cascade process, resulting in self-acceleration of the coagulation reaction.

Method used

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  • Water gel material with thrombin responsive thrombolysis capability and preparation method thereof
  • Water gel material with thrombin responsive thrombolysis capability and preparation method thereof
  • Water gel material with thrombin responsive thrombolysis capability and preparation method thereof

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preparation example Construction

[0038] The method for preparing thrombin-responsive thrombolytic materials provided by the present invention uses water-soluble vinyl molecules as monomers and thrombin substrate polypeptides with double bonds modified at both ends as cross-linking agents to form hydrocoagulants through free radical polymerization. glue.

[0039] The present invention will be further described below in conjunction with specific examples, but the present invention is not limited.

Embodiment 1

[0041] 25 μL of acrylamide solution with a concentration of 150 mg / mL, 5 μL of a polypeptide with a concentration of 50 mg / mL (the sequence is methacrylic acid-Gly-dPhe-Pro-Arg-Gly-Phe-Pro-Ala-Gly-Gly-Lys( Methacrylic acid)) solution, 2.5 μL tissue-type plasminogen activator (t-PA) solution with a concentration of 1 mg / mL, 0.5 μL N,N,N',N'-tetramethylethylenediamine ( TEMED), and 2 μL of ammonium persulfate solution with a concentration of 500 mg / mL (both solutions were prepared with phosphate buffered saline (PBS) with pH = 7.4) were mixed, and free radical polymerization was carried out at 25°C. After 20 min, the obtained The gel was soaked in PBS and washed for 3 hours to obtain a hydrogel material with thrombin-responsive thrombolytic function.

[0042] The process of releasing t-PA from the hydrogel under the action of thrombin was tested by isotope labeling. Such as figure 1 As shown, t-PA can be significantly released only in the presence of thrombin, and the release ...

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Abstract

The invention discloses a water gel material with thrombin responsive thrombolysis capability and a preparation method thereof, the water gel material with thrombin responsive thrombolysis capability is prepared as follows thrombin substrate peptide is used as a crosslinking agent, water gel is formed by free radical polymerization, meanwhile the water gel coats thrombolysis active molecules, and the thrombolysis active molecules only can significantly release and play fibrinolytic function. The material has the thrombin responsive thrombolysis capability, avoids the defect of a series of complications caused by the long-term exposure of reactive molecules of traditional thrombolysis materials, and has application prospect in the aspect of human implanted blood contact materials.

Description

technical field [0001] The invention relates to the fields of biomedical polymer materials and polymer chemistry, in particular to a material with responsive thrombolytic function and a preparation method thereof. Background technique [0002] The generation of thrombus induced by materials is one of the main reasons for the failure of human implanted blood contact devices. At present, the most important way to solve this problem is to introduce antithrombotic active molecules into materials, such as heparin and plasminogen, etc., and then realize Inhibition of the coagulation reaction by the material or dissolution of nascent thrombus. However, long-term exposure to antithrombotic active molecules will inevitably lead to dysfunction of the blood system, accompanied by serious complications such as tissue hemorrhage. An ideal antithrombotic active material should have thrombus responsiveness, that is, the antithrombotic mechanism should only be activated when the blood coag...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08F220/56A61L33/18A61L31/06A61L31/14A61L31/16
Inventor 陈红杜慧李丹
Owner SUZHOU UNIV
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