Three-gradient elution analysis method of glycosylated hemoglobin analyzer
A technology for glycosylated hemoglobin and hemoglobin, which is applied in the field of three-gradient elution analysis method of glycosylated hemoglobin analyzer, can solve the problems of high manufacturing and use cost of the instrument, increased complexity of the instrument, cross-contamination of the eluent, etc. The effect of simplified structure and precise separation
Inactive Publication Date: 2015-08-26
JIANGSU AUDICOM MEDICAL TECH
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Problems solved by technology
Due to the use of eluents with different concentrations in the machine, it will inevitably lead to an increase in the complexity of the instrument and a relative increase in the failure rate, and the cross-contamination of the eluent is serious, and the cost of manufacturing, maintenance, and use of the instrument is also high.
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[0029] exist figure 1 In the shown elution mechanism, three independent specific ion concentration eluents are set, namely: 3 is A eluent, 2 is B eluent, 1 is C eluent, and the three eluent containers are The outlet pipelines are respectively controlled by their respective solenoid valves, and can be circulated and transported sequentially to the chromatography column 6 made of cation exchange resin that absorbs the sample to be tested through the liquid supply port 5 under the action of the peristaltic pump 4 respectively. , the sample is eluted, and the eluted liquid is input into the colorimetric cell 7 for chromatographic analysis.
[0030] In the whole process, the glycosylated hemoglobin subfraction (HbA 1a+b ), and then B eluent 2 to elute glycosylated hemoglobin (HbA 1c ), followed by C eluent 1 to elute non-glycosylated hemoglobin (HbA 0 ), and finally the chromatography column 6 of the cation exchange resin is cleaned, equilibrated and regenerated by the eluent A ...
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The invention discloses a three-gradient elution analysis method of a glycosylated hemoglobin analyzer. The proportion of HbAlc in a clinical sample is analyzed with a cation exchange chromatography method, that is to say, glycosylated hemoglobin subcomponents HbAla+b (namely, HbAla and HbAlb) and HbAlc and non-glycosylated hemoglobin HbA0 adsorbed on cation exchange resin are eluted and separated by utilizing three elution solutions with specific ion concentrations in sequence, a separated sample is continuously subjected to colorimetric measurement in real time to obtain a three-gradient concentration and elution chromatography composite graph, and the proportion of the waveform area of HbAlc in the total waveform area of the three components is calculated to obtain the proportion of HbAlc in the hemoglobin. According to the method, elution operation links and consumable types are further simplified, and the reliability of equipment and the accuracy of detection are improved.
Description
technical field [0001] The invention relates to a detection method for analyzing material components by ion exchange and chromatography, in particular to a three-gradient elution analysis method for a glycated hemoglobin analyzer. Background technique [0002] The concentration of glycosylated hemoglobin in human blood is an important clinical indicator for the diagnosis of diabetes. Normal hemoglobin (Hb) will form various subcomponents after being glycosylated, but the most important subcomponent is HbA 1c , its structure is stable after its formation, and it has a life cycle of 90 to 120 days. By detecting this index, it can reflect the average blood sugar level of a diabetic patient for three months. Therefore, clinically, this indicator is generally used as an important basis for the screening, diagnosis and treatment of diabetic patients. [0003] At present, the determination of glycosylated hemoglobin mainly adopts ion exchange chromatography, and its principle is...
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IPC IPC(8): G01N30/06
Inventor 庄东宁
Owner JIANGSU AUDICOM MEDICAL TECH