Saccharomycetes culture medium and preparation method thereof
A culture medium and yeast technology, applied in the field of yeast culture medium and its preparation, can solve the problems of easy turbidity of the filtrate, complex production, unsuitability for large-scale yeast culture, etc., to speed up the growth and reproduction speed, and the preparation method is simple and easy , the effect of inhibiting the growth of miscellaneous bacteria
Active Publication Date: 2015-09-02
GUANGZHOU HUANYA COSMETIC SCI & TECH CO LTD
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Problems solved by technology
Wort culture medium is an excellent yeast medium, but there are many problems in the preparation of wort medium, such as complicated production, difficulty in controlling the self-saccharification of malt powder and the need for titration verification, and easy turbidity of filtrate, which is not suitable for production practice. Large-scale yeast culture
However, the single nutrient composition of the potato dextrose water medium leads to slow growth of the bacteria, which often cannot meet the needs of the rapid growth of the yeast population.
At the same time, the above two media also have a common and easy defect-it is easy to grow miscellaneous bacteria while cultivating yeast
Method used
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Embodiment 1 and 2
[0032] Yeast culture medium is made of the components of the mass percentage in the following table:
[0033]
[0034]
[0035] The preparation method of above-mentioned yeast culture medium comprises the steps as follows:
[0036] Each Chinese medicine powder was baked in an oven at 70°C for 24 hours, then pulverized in a Chinese medicine grinder at a rate of 18,000 rpm for 10 seconds, and the resulting powder was baked in an oven at 70°C for 24 hours, and then in a Chinese medicine grinder at a Grinding at 18000 rpm for 10 seconds; sieve the dried and pulverized medicinal powder with a flat sieve, take 100-200 mesh fine powder and mix evenly;
[0037] Weigh the Chinese medicine powder and other components in a beaker according to the formula, add water and stir to mix, heat to 90°C and stir for 5 minutes, filter out the insoluble matter; divide the above solution and sterilize it in an autoclave at 120°C for 20 minutes , naturally cooled to room temperature for later ...
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The invention relates to a saccharomycetes culture medium. The saccharomycetes culture medium consists of potato extracts, glucose, chloromycetin, traditional Chinese medicine powder, pH (potential of hydrogen) value regulating agents and water; and the traditional Chinese medicine powder at least comprises an optional combination of more than two of pipewort powder, radix polygonati officinalis powder, Chinese angelica powder, Chinese yam powder, radix ophiopogonis powder and tuckahoe powder. The invention also discloses a preparation method of the saccharomycetes culture medium. The saccharomycetes culture medium has the advantages that after being treated simply, the traditional Chinese medicine powder serves as a raw material of the saccharomycetes culture medium, and requirements of the environment-friendly and natural concept are met; materials are convenient to take, the preparation method is simple and practicable, and the saccharomycetes culture medium is suitable to be produced and used in batches; the survival rate of saccharomycetes is improved effectively, and growth and propagation speed is increased; and growth of infectious microbes can be restrained effectively, and growth of the saccharomycetes is not affected.
Description
technical field [0001] The invention relates to a microorganism culture medium, in particular to a yeast culture medium and a preparation method thereof. Background technique [0002] The culture medium is an artificially prepared nutrient matrix for the growth and reproduction of microorganisms or the accumulation of metabolites. It generally contains carbon sources, nitrogen sources, inorganic salts (including trace elements) and water. In nature, there are many kinds of microorganisms and various types of nutrients, and the requirements of different types of microorganisms for nutrient elements are quite different. When preparing the medium, the nutritional needs of the microorganisms should be considered first, and a highly targeted medium should be selected. [0003] Yeasts are single-celled fungi. Yeast is extremely rich in protein, carbohydrates, nucleic acids, minerals, B vitamins and organic acids. Yeast is used in many industries, such as in the food industry fo...
Claims
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IPC IPC(8): C12N1/16
CPCC12N1/16
Inventor 张怡
Owner GUANGZHOU HUANYA COSMETIC SCI & TECH CO LTD