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A coronoid cystic strain

A technology of coronoid loose capsule strains and seeds, applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of weak adaptability, slow fermentation start, unstable aroma, etc., and achieve the effect of strong adaptability

Active Publication Date: 2017-12-19
黄山水工茶业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the production process of Fuzhuan dark tea, the commonly used fermentation dominant bacteria is S. coronis, and the performance of S. coronis is closely related to the quality of dark tea products. The existing strains of S. coronoids have weak adaptability 1. The fermentation starts slowly, and the quality of the obtained black tea products is uneven, and the quality is difficult to be guaranteed, especially in terms of tea aroma, which has always had shortcomings such as unstable aroma and presence of miscellaneous gas

Method used

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  • A coronoid cystic strain

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Effect test

Embodiment 1

[0021] The culture method of coronoid loose capsule bacterial strain CGMCC10610 comprises the following steps:

[0022] Preparation of medium: Weigh 200g of peeled potatoes, cut into pieces, add water to boil, filter with gauze, then add 20% agar powder under heating conditions, continue heating and stirring, after the agar powder is dissolved, add glucose, stir Evenly, after cooling, add water to 1000mL, bandage, sterilize at 121°C for 20 minutes, and pour it into a plate after cooling; culture steps: under sterile conditions, streak the coronoid cystic strain CGMCC10610 on the plate, and the streaking is over Afterwards, the plates were inverted and placed in a constant temperature incubator at 28°C for 5 days.

Embodiment 2

[0024] The culture method of coronoid loose capsule bacterial strain CGMCC10610 comprises the following steps:

[0025] Preparation of medium: Weigh 200g of peeled potatoes, cut into pieces, add water to boil, filter with gauze, then add 15g of agar powder under heating conditions, continue heating and stirring, after the agar powder is dissolved, add glucose, and stir Evenly, after cooling, add water to 1000mL, bandage, sterilize at 121°C for 20 minutes, and pour it into a plate after cooling; culture steps: under sterile conditions, streak the coronoid cystic strain CGMCC10610 on the plate, and the streaking is over Afterwards, the plates were inverted and placed in a constant temperature incubator at 28°C for 7 days.

Embodiment 3

[0027] The preparation method for preparing the seed liquid of Saccharomyces coronoidis for dark tea comprises the following steps:

[0028] (1) Inoculate the coronoid cystic strain CGMCC10610 on a PDA plate, and culture it in a constant temperature incubator at 28°C until the plate is covered with colonies. Under aseptic conditions, add 10 mL of sterile distilled water to the plate covered with colonies, Scrape the yellow hyphae on the plate into sterile distilled water, use a syringe to absorb the turbid liquid in the plate, put it into a 50mL centrifuge tube, add sterile small glass beads, and centrifuge in a centrifuge for 5min (rotation: 3000r / min). After filtering through absorbent cotton, the spore suspension was obtained, and finally the concentration of the spore suspension was adjusted to 5.0×10 6 a / mL;

[0029] (2) get the tea raw material extract that concentration is 4%, as liquid culture medium after sterilization treatment, under aseptic condition, inoculate sp...

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Abstract

A coronoid loose capsule strain, the preservation number of which is CGMCC No.10610. The present invention also provides the application of the coronoid-losing capsule strain CGMCC10610 in the preparation of black tea. Compared with the existing coronoid sac strain, the coronoid sac strain CGMCC10610 has the following advantages: (1) The fermentation starts quickly, and the coronoid sac strain CGMCC10610 is inoculated on a PDA plate for activation, and an obvious yellow color can be grown in three days Mycelia, five days can be used for the preparation of first-level seed liquid; (2) strong adaptability, without adding any external energy, the coronoid sac strain CGMCC10610 can grow and reproduce rapidly only by relying on tea extract, and the fermented bacteria The dry weight of the silk is as high as 0.6404g / 100mL, while the dry weight of the fermented mycelia of the existing strain CGMCC10610 is only 0.3743g / 100mL; The tea products produced are rich in aroma, stable and free of miscellaneous gases, and the tea products have a mellow taste, neither bitter nor astringent.

Description

technical field [0001] The invention belongs to the application field of microorganisms, and in particular relates to a coronoid loose capsule strain. Background technique [0002] Dark tea is a kind of special tea made by microbial fermentation during processing. It has significant functions of reducing fat and blood sugar, so it is more and more popular among the people. [0003] In the production process of Fuzhuan dark tea, the commonly used fermentation dominant bacteria is S. coronis, and the performance of S. coronis is closely related to the quality of dark tea products. The existing strains of S. coronoids have weak adaptability 1. Fermentation start is slow, the quality of gained black tea product is uneven, and quality is difficult to be guaranteed, especially always have the shortcomings such as fragrance intensity instability, miscellaneous gas aspect tea fragrance. Contents of the invention [0004] The technical problem to be solved by the present invention...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23F3/08C12R1/645
Inventor 高学玲卢恒谦岳鹏翔周燕丽
Owner 黄山水工茶业有限公司
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