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Microbial decomposing agent and its preparation and application in organic material fermentation and decomposing

A microbial decomposing agent and organic material technology, applied in the direction of fixing on/in organic carriers, bio-organic part treatment, microorganism-based methods, etc., can solve problems such as single use, low fermentation efficiency, and long fermentation cycle , to achieve the effects of simple preparation method, reduction of plant diseases, and quick start of fermentation

Active Publication Date: 2022-06-28
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many technologies and products related to decomposing bacteria agents, but most of them have the following problems: First, the fermentation efficiency is not high
The performance is slow start, slow deodorization, large amount of addition, and long fermentation cycle
Second, it is not resistant to low temperature
Most of the strains used are medium-temperature, suitable for use at an ambient temperature above 20°C. If the temperature is low, the fermentation will take a long time to start or will not ferment, and it will be difficult to carry out fermentation production in winter
Three is a single purpose
Most of them are only used for livestock and poultry manure fermentation or straw fermentation, resulting in high R&D and production costs
The main reasons for the above-mentioned problems are: firstly, the composition of the strains of the starter is unreasonable, and some strains are simple combinations without considering the functional complementarity and mutual symbiosis between the constituent strains, resulting in low fermentation efficiency, especially at low temperature. slow or no fermentation
Second, the composition of the strain is a simple combination with a single function
[0007] The applicant has not seen any literature reports similar to the technical solutions and technical effects of the present application in the prior art

Method used

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  • Microbial decomposing agent and its preparation and application in organic material fermentation and decomposing
  • Microbial decomposing agent and its preparation and application in organic material fermentation and decomposing
  • Microbial decomposing agent and its preparation and application in organic material fermentation and decomposing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] 10 parts of compound original bacteria powder; 30 parts of organic material carrier; 20 parts of inorganic carrier;

[0064] The compound original fungus powder is composed of Wickerhamomyces anomalus with the preservation number of CGMCC No.20654 and Rhizopus oryzae with the preservation number of CGMCC No.3.5085. Abnormal Wickham yeast: Rhizopus oryzae = 1.5: 0.5, the number of viable bacteria in the microbial decomposing agent is greater than 2 × 10 8 cfu / g.

[0065] The strain in this example, yeast Wickham abnormality, was isolated from the feces of pig farming. The strain was deposited in the General Microbiology Center of the China Microorganism Culture Collection and Management Committee on September 15, 2020, and was named after classification. It is Wickerhamomyces anomalus, the deposit number is CGMCC No. 20654, and the address of the deposit institution is located at the Institute of Microbiology, Chinese Academy of Sciences (CGMCC), No. 3, No. 1, Beichen W...

Embodiment 2

[0083] The difference between this embodiment and Embodiment 1 is:

[0084] 30 parts of compound original bacteria powder; 50 parts of organic material carrier; 50 parts of inorganic carrier;

[0085] Abnormal Wickham yeast in compound original powder: mass fraction of Rhizopus oryzae = 2.5: 1.5, and the number of viable bacteria in microbial decomposing agent is greater than 2 × 10 8 cfu / g.

[0086] The organic material carrier is selected from lignite powder.

[0087] The inorganic carrier is selected from talc.

[0088] The period in the liquid seed preparation conditions of steps A and B in the preparation method of the microbial decomposing agent is 72 hours.

[0089] In the preparation method of the microbial decomposing agent, the fermentation or culturing conditions of the solid curved plate in steps A and B are: culturing in an environment of 30° C. for 72 hours.

[0090] The rest of the contents are the same as in Example 1.

Embodiment 3

[0092] The difference between this embodiment and Embodiment 1 is:

[0093] 20 parts of compound original bacteria powder; 40 parts of organic material carrier; 35 parts of inorganic carrier;

[0094] Abnormal Wickham yeast in the composite original powder: the mass fraction of Rhizopus oryzae = 2:1, and the number of viable bacteria in the microbial decomposing agent is greater than 2 × 10 8 cfu / g.

[0095] The organic material carrier is selected from bran powder.

[0096] Said inorganic carrier is selected from medical stone.

[0097] The period in the liquid seed preparation conditions of steps A and B in the preparation method of the microbial decomposing agent is 60 hours.

[0098] In the preparation method of the microbial decomposing agent, the fermentation or culturing conditions of the solid curved plate in steps A and B are: culturing in an environment of 30° C. for 60 hours.

[0099] The rest of the contents are the same as in Example 1.

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Abstract

The invention belongs to the preparation of a microbial decomposing agent, in particular to a microbial decomposing agent and its preparation and application in the fermentation and decomposing of organic materials. The composite original bacterial powder is made from Wickerhamomyces anomalus CGMCC No.20654 and Rhizopus oryzae CGMCC No.3.5085, and the microbial decomposing agent is made by mixing the composite original bacterial powder with organic material carriers and inorganic carriers. The invention solves the problems in the prior art that the strain starts slowly or does not start at low temperature, has single use, and the like. The method can be started at an ambient temperature of 15° C., effectively decomposes and degrades crop stalks and livestock and poultry manure, and has a fast decomposing speed and a simple preparation method.

Description

technical field [0001] The invention belongs to the preparation of a microbial decomposing agent, in particular to a microbial decomposing agent that can be started at a lower ambient temperature, and its preparation and application in the fermentation and decomposing of organic materials. Background technique [0002] A large amount of organic waste will be produced in aquaculture, planting, sewage purification, food processing, pharmaceutical and other industries. Improper handling will lead to pollution of the surrounding environment, groundwater and farmland, and adversely affect production, life and people's health. Using high-temperature aerobic fermentation technology, it can generate a large amount of biological heat, kill the eggs, pathogenic bacteria, grass seeds, etc., and at the same time convert macromolecular organic matter such as cellulose, lignin, and protein into humus, amino acids, etc., and release nitrogen. Phosphorus, potassium and medium and trace elem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/14C12N11/02C05F17/20C05F17/10C05G1/00C12R1/645C12R1/845
CPCC12N1/16C12N1/14C12N11/02C05F17/20C05F17/10C05D9/00C05D5/00C05F11/02C05F11/08C05F5/002Y02W30/40
Inventor 王占武张翠绵胡栋王旭贾楠彭杰丽马佳
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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