Anthurium adndraeanum pollen culture method

A technology of pollen culture and anthurium, applied in the field of plant tissue culture, to achieve the effects of innovation of germplasm resources, short time to embryo formation, and great practical value

Active Publication Date: 2015-09-16
XISHAN XIANFENG FAMILY FARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at present problem,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for cultivating anthurium pollen, comprising the following steps,

[0020] 1) Inoculate 1000 grains of pollen from the mononuclear marginal stage into a callus induction medium, and culture to obtain a callus. The callus induction medium: WPM+NAA 0.3mg / L+6-BA 0.2mg / L L+2.4-D 0.4mg / L+Gibberellin 2.2mg / L+Agar 1500mg / L+Sucrose 600mg / L;

[0021] 2) inoculate the differentiation medium, cultivate and obtain test tube seedlings, and the described differentiation medium is WPM+6-BA 0.4mg / L+NAA 0.1mg / L+ZT 0.6mg / L+600mg / L sucrose;

[0022] 3) Inoculate the rooting medium and screen to obtain single plants. The rooting medium is 1 / 2WPM+IBA 0.1mg / L+NAA0.6mg / L+agar 600mg / L+sucrose 1400mg / L+activated carbon mass ratio 0.1%.

[0023] The culture conditions adopt the method of combining light culture and dark culture. The light culture light source adopts fluorescent lamps. The daytime temperature in the culture room is 28°C, the lighting is 12h, and the light intensity is 1...

Embodiment 2

[0026] A method for cultivating anthurium pollen, comprising the following steps,

[0027] 1) Inoculate the pollen at the marginal stage of mononuclei into the callus induction medium, and culture to obtain the callus. The callus induction medium: WPM+NAA0.5mg / L+6-BA0.4mg / L+ 2.4-D 0.7mg / L+ gibberellin 2.5mg / L + agar 1700mg / L+ sucrose 800mg / L;

[0028] 2) inoculate the differentiation medium, cultivate and obtain test tube seedlings, and the described differentiation medium is WPM+6-BA 0.6mg / L+NAA 0.3mg / L+ZT 1mg / L+800mg / L sucrose;

[0029] 3) Inoculate the rooting medium and screen to obtain single plants. The rooting medium is 1 / 2WPM+IBA 0.3mg / L+NAA 0.8mg / L+agar 800mg / L+sucrose 1600mg / L+activated carbon mass ratio 0.3%.

[0030] The culture conditions adopt the combination of light culture and dark culture. The light culture light source adopts fluorescent lamps. The daytime temperature in the culture room is 30°C, the lighting is 12h, and the light intensity is 20μmol.m -2 ...

Embodiment 3

[0033] A method for cultivating anthurium pollen, comprising the following steps,

[0034] 1) Inoculate the pollen at the marginal stage of mononuclei into the callus induction medium, and culture to obtain the callus. The callus induction medium: WPM+NAA 0.4mg / L+6-BA 0.3mg / L+ 2.4-D 0.6mg / L+ gibberellin 2.4mg / L + agar 1600mg / L+ sucrose 700mg / L;

[0035] 2) Inoculate the differentiation medium, cultivate and obtain the test tube seedlings, the differentiation medium is WPM+6-BA 0.5mg / L+NAA 0.2mg / L+ZT 0.8mg / L+700mg / L sucrose;

[0036] 3) Inoculate the rooting medium and screen to obtain single plants. The rooting medium is 1 / 2WPM+IBA 0.2mg / L+NAA0.7mg / L+agar 700mg / L+sucrose 1500mg / L+activated carbon mass ratio 0.2%.

[0037] The culture conditions adopt the combination of light culture and dark culture. The light culture light source adopts fluorescent lamps. The daytime temperature in the culture room is 29°C, the lighting is 12h, and the light intensity is 17μmol.m -2 .s -1 ...

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PUM

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Abstract

The invention relates to an anthurium adndraeanum pollen culture method. The method comprises the following steps: an anther at a mid-late uninucleate stage is adopted and is inoculated to a callus induction medium, and callus is obtained by culturing; the callus is inoculated to a differentiation medium, such that test tube seedlings are obtained; the test tube seedlings are inoculated to a rooting medium, and screening is carried out, such that single plants are obtained. With the above culturing manner, an anthurium adndraeanum pollen callus induction rate reaches 88%, a rooting rate reaches 94%, and a transplanting survival rate reaches 90%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to an anthurium pollen culture method. Background technique [0002] Anthurium adndraeanum is a perennial evergreen herb of Araceae Anthurium, which can be used as potted flowers and cut flowers. It is one of the most popular and precious flowers in the world today, with high ornamental value and economic value. [0003] Anthurium is an important tropical cut flower, the inflorescence of the Buddha flame, the buds of the Buddha flame are huge, thick and waxy, and the colors are red, pink, white, green, two-color and so on. Its bright color, unique shape, wide range of applications, and high economic value are high-end tropical cut flowers and potted flowers that are developing rapidly in the world and are in great demand. In the tropical rainforest where Anthurium is native, Anthurium can be propagated by seeds, but it takes a long time to enter flowering. Branch propagation is the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 于永军
Owner XISHAN XIANFENG FAMILY FARM
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