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A cultivation method for carassius auratus gynogenesis fries

A technology of gynogenesis and crucian carp, applied in the field of fish genetics and breeding, can solve the problems of diploid wild crucian carp breeding application blank

Active Publication Date: 2015-09-16
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding application of diploid wild crucian carp gynogenesis is still blank

Method used

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  • A cultivation method for carassius auratus gynogenesis fries
  • A cultivation method for carassius auratus gynogenesis fries
  • A cultivation method for carassius auratus gynogenesis fries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Identification of crucian carp ploidy: Through a large number of screening crucian carp microsatellite markers, we obtained 4 microsatellite markers that can clearly distinguish diploid and triploid crucian carp (see Table 1 for marker names and primer sequences; HLJY018, HLJY029, HLJY049 and YJ0004), the ploidy identification rate of these four microsatellite markers in crucian carp with known ploidy reached 100%. Four microsatellite markers were used to identify the ploidy of the wild crucian carp population from Zhangdu Lake. The ploidy determination method was as follows: the individual crucian carp individuals with three alleles in any of the above microsatellite markers in the genotyping results were It is considered to be triploid crucian carp, and individuals who have never appeared three alleles are considered to be diploid crucian carp. The steps of DNA extraction and microsatellite typing are as follows:

[0033](1) The caudal fins of wild crucian carp in...

Embodiment 2

[0042] According to the identification method in Example 1, another pair of diploid parents of crucian carp was selected, and only slightly changed the conditions of sperm treatment and cold shock treatment of fertilized eggs.

[0043] 1. Inactivation of sperm genetic material: Dilute the semen 6 times with pre-cooled Hanks solution, adjust the thickness to 1.5mm, place it on an ice box, and then irradiate it at 17cm under a UV lamp for 22min.

[0044] 2. Chromosome doubling induced by meiosis: mix the eggs of crucian carp with genetically inactivated sperm, add aerated water to start insemination, and place the fertilized eggs in pre-cooled aerated water at 6.5°C after 3 minutes, and perform cold shock treatment for 25 minutes , and then incubated at room temperature. The obtained fry were compiled as crucian carp gynogenetic family 2.

[0045] 3. Early growth traits and genetic analysis: The induced normal diploid gynogenetic offspring were cultured indoors, 66 gynogenetic ...

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Abstract

The invention provides a cultivation method for carassius auratus gynogenesis fries. The method comprises the steps of selecting sexually mature diploid carassius auratus parents and collecting sperm and ovums; performing ultraviolet irradiation on Hanks liquid-diluted sperms to inactivate genetic materials thereof; performing artificial insemination on the inactivated sperms and the ovums; restraining the discharge of second polar bodies of meiosis by using a cold shock method to double genomes of the ovums and thus to cultivate viable carassius auratus gynogenesis diploid fries. Microsatellite marker identification proves that the genetic materials of all the gynogenesis fries are from the female parent; morphological measurement indicates part of the gynogenesis fries has obvious early growth dominance. The method has the advantages of simple operation, high efficiency, stability and high practicality.

Description

technical field [0001] The invention belongs to the field of fish genetics and breeding, in particular to a method for inducing and cultivating crucian carp gynogenetic fry using homologous sperm. Background technique [0002] Gynogenesis is the use of genetically inactivated sperm to activate the egg. When the sperm without genetic information penetrates into the egg, the egg can develop and divide. The sperm nucleus of this genetically inactivated sperm does not combine with the female pronucleus, and the embryo Development is regulated by genes carried by a single set of chromosomes in the female nucleus (Wu Qingjiang et al. Fish Genetics and Breeding Project. Shanghai: Shanghai Science and Technology Press. 1999.). Artificially induced gynogenesis is of great significance to fish sex control, parthenoculture, rapid establishment of pure lines, and accelerated breeding of improved species. This technology has been widely used in the research and practice of many species o...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCA01K61/00A01K61/90Y02A40/81
Inventor 童金苟王新华俞小牧刘海洋付北德冯秀
Owner INST OF AQUATIC LIFE ACAD SINICA
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