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Method for cultivating transgenic plant with increased content of isoflavone and condensed tannin and special plasmid

A technology of transgenic plants and condensed tannins, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and plant products, and can solve problems such as low condensed tannin content

Active Publication Date: 2015-09-16
CHINA AGRI UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of condensed tannins in alfalfa leaves is extremely low, and effective means are urgently needed to increase the content of condensed tannins in the ingested part of alfalfa

Method used

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  • Method for cultivating transgenic plant with increased content of isoflavone and condensed tannin and special plasmid
  • Method for cultivating transgenic plant with increased content of isoflavone and condensed tannin and special plasmid
  • Method for cultivating transgenic plant with increased content of isoflavone and condensed tannin and special plasmid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1. Obtaining and Identification of Transgenic Alfalfa High in Isoflavones and Condensed Tannins

[0062] 1. Preparation of plasmid pDES200-A-B-C

[0063] Plasmid 1 was synthesized. The nucleotide sequence of the plasmid was a double-stranded DNA molecule shown in Sequence 1 of the Sequence Listing, which was named plasmid pDES200-A-B-C. The schematic diagram of the T-DNA region of plasmid pDES200-A-B-C is shown in figure 1 . There are three expression cassettes in the plasmid pDES200-A-B-C, named as expression cassette A, expression cassette B and expression cassette C, respectively.

[0064] The reverse complementary sequence of the expression cassette A is shown in the 75th to 1819th positions from the 5' end of the sequence listing sequence 1, wherein the reverse complementary sequence of the NOS promoter is in the 1513th to 1819th positions from the 5' end of the sequence listing sequence 1 , the 337th to 1506th are the reverse complementary sequence of t...

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Abstract

The invention discloses a method for cultivating a transgenic plant with increased content of isoflavone and condensed tannin and a special plasmid. The method for cultivating the transgenic plant with increased content of isoflavone and condensed tannin comprises the following steps: importing nucleic acid molecules for coding proteins A, nucleic acid molecules for coding proteins B and nucleic acid molecules for coding proteins C into a receiver plant, thereby obtaining the transgenic plant of which the content of isoflavone and / or condensed tannin is higher than that of the receiver plant. The experiment proves that the content of the isoflavone and / or condensed tannin in the transgenic plant obtained by the method provided by the invention is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for cultivating transgenic plants with increased isoflavone and condensed tannin contents and a special plasmid. Background technique [0002] Alfalfa is an important leguminous herbage, which has the characteristics of good palatability, high protein content, high quality, and high biomass production. It is widely used in animal husbandry as feed for ruminants such as cattle and sheep. However, due to the high protein content of alfalfa, ruminants tend to produce a lot of foam during digestion, and microbial fermentation in the rumen produces methane gas, which can easily lead to flatulence and even death. Studies have found that a certain amount of condensed tannins can effectively prevent the occurrence of flatulence, and condensed tannins have the ability to resist oxidation and scavenge free radicals. However, the content of condensed tannins in alfalfa lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84C12N5/10C12N1/21A01H5/00
Inventor 王涛苟蓝明董江丽
Owner CHINA AGRI UNIV
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