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Invisible polypeptide with good non-specific protein absorption resistance performance and preparation method thereof

A non-specific, protein-based technology, applied in the field of stealth polypeptides and their preparation, to achieve stealth performance and good anti-protein adsorption performance

Active Publication Date: 2015-09-23
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this material shows good resistance to non-specific protein adsorption, its non-biodegradability problem still exists

Method used

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  • Invisible polypeptide with good non-specific protein absorption resistance performance and preparation method thereof
  • Invisible polypeptide with good non-specific protein absorption resistance performance and preparation method thereof
  • Invisible polypeptide with good non-specific protein absorption resistance performance and preparation method thereof

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Embodiment Construction

[0045] 1. Condensation

[0046] δ-(α-Benzyl-N ε Synthesis of -benzyloxycarbonyl-L-lysyl)-α-tert-butyl-N-tert-butoxycarbonyl-L-aspartic acid (4)

[0047] Dissolve H-Lys(Z)-OBzl·HCl (1, 6.10g, 15.0mmol) in 30mL of anhydrous DMF, add 2.5mL of TEA (18.10mmol), react with stirring for 20min, filter; then BOC-ASP -O t Bu (3, 4.34g, 15.0mmol), EDC·HCl (5.75g, 30.0mmol), HOBt (4.04g, 30mmol) were added to the filtrate, and after nitrogen protection for 30min, the reaction was sealed at room temperature for 48h; Most of the DMF was distilled off to obtain a yellow oil; the yellow oil was dissolved in ethyl acetate, washed successively with 10wt.% citric acid solution, saturated sodium bicarbonate solution, and saturated brine, and the organic phase was washed with anhydrous Dry over sodium sulfate, remove the solvent by rotary evaporation, and separate by silica gel column chromatography (mobile phase: dichloromethane / ethyl acetate=3:1, volume ratio) to obtain white solid (4) (7.80 ...

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Abstract

The invention discloses an invisible polypeptide with a good non-specific protein absorption resistance performance and a preparation method thereof. The structural formula of the invisible polypeptide with the good non-specific protein absorption resistance performance is (please see the formula in the instruction). The invisible polypeptide is formed by condensation polymerization of dipolymer by covalent linkage of gamma-carboxyl of aspartic acid and alpha-amino of lysine, and the problem of biology non-degradability when the equivalent and uniform distribution electric charges needed by non-specific protein absorption materials and methacrylic acid and polyethylene glycol materials serve as drug carriers is solved.

Description

1. Technical field [0001] The invention relates to a polypeptide and a preparation method thereof, in particular to a stealth polypeptide with good anti-nonspecific protein adsorption performance and a preparation method thereof. 2. Background technology [0002] One problem that is still not fully resolved for biomaterials is non-specific protein adsorption on the material surface. It is one of the main factors leading to thrombosis, inflammatory responses, and even failure of biomaterials. Polyethylene glycol (PEG) is widely used in the field of biomedical materials, but it is easily oxidized and even degraded under the condition of long-term contact with physiological blood. It is generally believed that the stability of zwitterionic anti-non-specific protein adsorption materials is better than that of PEG, and the most typical ones are carboxybetaine (CBMA), sulfobetaine (SBMA) and phosphorylcholine (MPC). All belong to methacrylate materials. However, when PEG and me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/02C07K14/00C07K1/06C07K1/02
Inventor 杨庆华郑磊张新晴王强盛
Owner HEFEI UNIV OF TECH