Immunity magnetic bead test strip for testing tumor marker CA72-4 and preparing method thereof

Abstract

The invention provides an immunity magnetic bead test strip for testing tumor marker CA72-4 and a preparing method thereof. The immunity magnetic bead test strip comprises a sample pad, a connecting pad, a nitrocellulose film, a water absorbing pad and a backing board. The sample pad, the connecting pad, the nitrocellulose film and the water absorbing pad are stacked in order on the backing board. The stacked parts along the length of the backing board are 0.5 to 2 mm in length. An immunochromatographic method for use is a double-antibody sandwich method; two antibodies are an anti-CA72-4 monoclonal antibody and anti-CA72-4 monoclonal antibody B72.3. The preparing method mainly relates to the preparation of nano magnetic bead probes. After the immunochromatography with the strip, a magnetic intensity tester is used to analyze and acquire CA72-4 quantitative results; the preparing method is simple and fast and is higher in sensitivity and specificity.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to an immunomagnetic bead test strip for detecting tumor marker CA72-4 and a preparation method thereof. Background technique [0002] Gastric cancer is one of the most common malignant tumors with a high fatality rate in the world, and it is also one of the most common malignant tumors in my country. The incidence of gastric cancer has remained high. According to data, there are 400,000 new gastric cancer patients and 300,000 deaths every year in my country, and the proportion of young people suffering from gastric cancer is increasing, and about 15% of the patients are young people under the age of 40. In China, the early diagnosis rate of gastric cancer is less than 10%. When patients are diagnosed with gastric cancer, they have already reached the stage of advanced gastric cancer. The 5-year survival rate reaches 80%-90%. Therefore, the development of early gas...

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Problems solved by technology

[0008] 1. At present, the clinical methods for detecting CA72-4 are mainly traditional immunological detection methods, such as electrochemiluminescence, ELISA, and enzyme-linked fluorescent immunoassay system. The operation process requires special laboratories and professionals for detection and operation. The steps are complicated and the detection time is long, usually 18-24 hours, which is not suitable for early screening of cancer patients

[0009] 2. Among the existing rapid detection methods, there is no report on the use of immunochromatographic detection methods to detect the tumor marker CA72-4 in the body's serum

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