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Method for generating emulsion in micro-channel

A microchannel and microfluidic channel technology, which is used in the field of emulsion generation in microchannels, can solve the problems of inability to realize the size and volume of the emulsion, as well as the dispersion and precise control.

Active Publication Date: 2015-09-30
CHONGQING UNIV
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  • Application Information

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Problems solved by technology

The current emulsion preparation process includes colloidal grinding, rotor stator system and membrane emulsification method, etc., but these methods cannot achieve precise control of the size, volume and dispersion of the emulsion, and achieve efficient and sensitive encapsulation and release of reagents inside the emulsion. control

Method used

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  • Method for generating emulsion in micro-channel
  • Method for generating emulsion in micro-channel

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Embodiment Construction

[0021] Below in conjunction with accompanying drawing and embodiment the present invention will be further described:

[0022] like figure 1 , figure 2 As shown, this embodiment includes the following steps:

[0023] a. Make a microfluidic channel mold on the silicon wafer, pour the prepared polydimethylsiloxane solution on the silicon wafer, and then put it on the heating plate to bake. A piece of polydimethylsiloxane 5 is torn off from the silicon chip, then forms microchannel on the surface of the first piece of polydimethylsiloxane 5, and this microchannel comprises primary emulsification inlet channel 8a, secondary emulsion The inlet channel 8b and the emulsion forming channel 8c, the inlet end of the primary emulsification inlet channel 8a, the inlet end of the secondary emulsion inlet channel 8b and the inlet end of the emulsion forming channel 8c are all provided with an inlet hole 1a and a microfluidic chip 1 External communication, the outlet end of the primary e...

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Abstract

The invention discloses a method for generating emulsion in a micro-channel. The method comprises the following steps: a, forming the micro-channel in the surface of a first piece of polydimethylsiloxane; b, sticking the first piece of polydimethylsiloxane with a second flat piece of polydimethylsiloxane together; c, vertically putting a laser device on one side of the second piece of polydimethylsiloxane; d, respectively injecting inner-layer liquid and outer-layer liquid of the emulsion from inlet ends of an emulsification inlet channel and an emulsion forming channel; e, starting the laser device so that the laser device is used for heating an outlet end of the primary emulsification inlet channel; and f, forming emulsion drops in the emulsion forming channel, and pushing the emulsion drops by liquid to a liquid outlet hole. According to the method, the emulsion is generated by adopting the double channels which are vertical to each other; and the laser device is used for heating so that the changes of emulsion size, generation speed and frequency, dispersity and the like are realized.

Description

technical field [0001] The invention particularly relates to a method for generating emulsion in a microchannel, specifically controlling the generation of emulsion in the microchannel through laser technology. Background technique [0002] In recent years, the emerging discipline of microelectromechanical systems, life sciences and analytical sciences—biological microelectromechanical systems, also known as microfluidic chips, lab-on-a-chip or micro-total analysis systems, has developed rapidly. It has a huge market space and can be widely used in life sciences, medicine, chemistry, new drug development, food and environmental hygiene supervision and other fields. The microfluidic chip is to miniaturize the functions of a traditional analytical laboratory to a small chip to build a chemical or biological laboratory. Compared with traditional analytical methods, the microfluidic chip has a fast response speed; less consumption of samples and reagents; The system has fewer e...

Claims

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Application Information

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IPC IPC(8): B01F3/08B01J13/00
Inventor 陈蓉程潇王宏廖强朱恂丁玉栋叶丁丁李俊黄云付乾
Owner CHONGQING UNIV
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