Method for preparing anti-virus molecularly imprinted polymer

A molecular imprinting and anti-virus technology, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of poor imprinting effect, virus variability, affecting imprinting effect, etc., to ensure specific recognition ability and simple regeneration method. , the effect of good economic benefits

Inactive Publication Date: 2015-09-30
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

[0005] Dopamine can undergo self-polymerization in alkaline solution (pH>8.5). The dopamine self-polymerization system is a water-soluble synthetic system without the use of organic solvents. It is suitable for the preparation of artificial antibodies for biological macromolecules such as proteins, but this system has not been used for the preparation of viruses. There are two main reasons for MIP: (1) The thickness of the dopamine self-polymerization film is between 10nm and 50nm, and the diameter of most viruses is greater than 50nm. The virus and the dopamine self-polymerization system are directly mixed for self-polymerization, and the imprinting effect is not good. (2) The self-polymerization of dopamine needs to be carried out under alkaline (pH>8.5) conditions, and the virus is easily denatured under alkaline conditions, which affects the effect of imprinting

Method used

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  • Method for preparing anti-virus molecularly imprinted polymer
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  • Method for preparing anti-virus molecularly imprinted polymer

Examples

Experimental program
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Embodiment 1f2

[0043] Example 1f2 Preparation of phage antiviral molecularly imprinted polymer (MIP)

[0044] Step 1: Surface modification of the carrier

[0045] Dissolve ammonium persulfate and dopamine in a Tris-HCl buffer solution with a pH value of 7.5 at a molar ratio of 2:1 to form a dopamine buffer; add silica microspheres as a carrier material to part of the dopamine buffer, and expose Slowly stir in the air to react, centrifuge and discard the reaction solution after 12 hours to obtain a dopamine-silicon dioxide complex;

[0046] In the reaction solution, the concentrations of dopamine and ammonium persulfate were 2 mg / mL and 1.5 mg / mL respectively, and the concentration of silica microspheres was 15 mg / mL;

[0047] Step 2: Combination of virus and carrier

[0048] Mix the dopamine-silica complex obtained in step 1 with another part of the dopamine buffer in step 1, and then add the target virus f2 phage so that the concentration of the target virus in the buffer is 10 8 pfu / mL,...

Embodiment 2

[0052] Repeat Example 1 by the same steps as described, the difference is that ammonium persulfate is not added in step one.

Embodiment 3

[0054] Repeat Example 1 according to the same steps as described, the difference is that the concentrations of dopamine and ammonium persulfate are 2 mg / mL and 1 mg / mL respectively, and the molar ratio is 3:1.

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Abstract

The invention discloses a method for preparing an anti-virus molecularly imprinted polymer. The method comprises the steps of modifying dopamine nanofilm on a carrier material, then combining a target virus with the carrier material modified with the dopamine nanofilm, forming a virus-dopamine-carrier material compound, eluting the target virus from the compound, and obtaining the corresponding anti-virus molecularly imprinted polymer. According to the anti-virus molecularly imprinted polymer, imprinted holes left after the virus is eluted can be utilized, the target virus is combined in a specific mode, and the target virus is prevented from multiplication and infecting a host; the anti-virus molecularly imprinted polymer has the advantages of being good in using specificity, friendly in service environment and simple in regenerate, and a good using prospect in the biological immunity field is achieved.

Description

technical field [0001] The invention belongs to the technical field of biological immunity, and more specifically relates to a preparation method of an antiviral molecularly imprinted polymer. Background technique [0002] Many human diseases are caused by viruses. Immunization and antiviral drugs are two methods of antiviral treatment, and immunization is currently the most effective way to control viral infections. Immunization methods include immunization and passive immunization with antibody-containing serum. However, immune preparations such as vaccines and immunoglobulins are proteins, and their preparation often requires live animals. The preparation process is complicated and sensitive to temperature, ions, and pH, and is not easy to store . In addition, protein preparations are prone to cause allergic reactions, and some people cannot use them. Due to these characteristics, some new and highly lethal viruses, such as Ebola virus, HIV virus, etc., still lack suita...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G73/02C08J9/26B01J20/26B01J20/30
Inventor 吕斌刘燕婕石云刘飞罗密芳
Owner HUAZHONG UNIV OF SCI & TECH
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