Molecular genetic SNP marker related to sow oestrous symptoms and detection method and application thereof

A sow estrus and molecular marker technology is applied in the field of molecular genetic SNP marker and its detection, which can solve the problems of decreased reproductive efficiency of pig farms, difficulty in estrus identification, and inconspicuous estrus symptoms.

Active Publication Date: 2015-10-28
博瑞迪生物技术(海南省)有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

This has led to an increase in sow litter size, but its estrus symptoms are becoming less and less obvious, and more recessive estrus appears, making estrus identification very difficult, and it is necessary to use test boars to test estrus repeatedly or other techniques The method can accurately identify the estrus, and the breeding efficiency of the pig farm is greatly reduced.

Method used

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  • Molecular genetic SNP marker related to sow oestrous symptoms and detection method and application thereof
  • Molecular genetic SNP marker related to sow oestrous symptoms and detection method and application thereof
  • Molecular genetic SNP marker related to sow oestrous symptoms and detection method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Collection of test samples

[0031] The test pigs came from Jiangsu Yongkang Agriculture and Animal Husbandry Co., Ltd. in Jintan City, Changzhou, China. The ear tissues of 100 “Landrace × Large White” hybrid sows were collected, put into 2mL centrifuge tubes containing 75% alcohol, and kept at -20°C save. Collect 5mL of blood from the anterior vena cava of each sow, let it stand at room temperature for 2h, centrifuge at 3000r / min for 5min, take the supernatant serum in a 1.5mL centrifuge tube, and store at -20°C.

Embodiment 2

[0033] 1. Genomic DNA extraction from 100 sows

[0034] The DNA samples of the pig population used in the present invention all adopt the traditional phenol-chloroform method to extract, and the required reagents include:

[0035] Lysate laboratory equipment

[0036] Proteinase K (Germany MERCK Biotechnology Co., Ltd.)

[0037] Tris Saturated Phenol (Beijing Suolaibao Biotechnology Co., Ltd.)

[0038] Tris Saturated Phenol: Chloroform: Isoamyl Alcohol (25:24:1) (Beijing Suo Laibao Biotechnology Co., Ltd.)

[0039] Chloroform (Jiangsu Yonghua Fine Chemicals Co., Ltd.)

[0040] Absolute ethanol (Guangdong Guanghua Technology Co., Ltd.)

[0041] 3M Sodium Acetate (Beijing Suolaibao Biotechnology Co., Ltd.)

[0042] The specific steps are as follows:

[0043] Take a soybean-sized tissue sample, cut it into pieces as much as possible and put it into a 2ml centrifuge tube;

[0044] Add 800 μL of lysate (provided by yourself), and 30 μL of proteinase K (40 mg / ml);

[0045] Pl...

Embodiment 3

[0064] Detection of Peripheral Blood Estrogen and Progesterone Concentration of 100 Sows

[0065] The detection of the concentration of serum estrogen and progesterone in the peripheral blood of pig population used in the present invention is detected by radioimmunoassay.

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Abstract

The invention discloses a molecular genetic SNP marker related to sow oestrous symptoms and a detection method and application thereof. The SNP marker is located on the nucleotide sequence of the gene CYP17A1 on the sow chromosome number 14, and the SNP marker locus, namely rs80850169, is the nucleotide locus g.123778229 on the sow chromosome number 14 in the reference sequence of revision 10.2 from the international genome. Through the molecular genetic SNP marker, it is found that the SNP locus is significantly related to the estrogen concentration in serum of a sow which is in oestrum. By means of the application of the molecular marker in the sow oestrus detection, the novel molecular genetic marker is provided for sow oestrus detection and breeding.

Description

technical field [0001] The invention belongs to the technical field of animal molecular breeding, and relates to molecular genetic SNP markers related to estrous symptoms of sows, a detection method and application thereof. Background technique [0002] In pig breeding programs, estrus symptoms in sows are rarely used as a breeding indicator. This has led to an increase in sow litter size, but its estrus symptoms are becoming less and less obvious, and more recessive estrus appears, making estrus identification very difficult, and it is necessary to use test boars to test estrus repeatedly or other techniques means can accurately carry out estrus identification, and the breeding efficiency of pig farms is greatly reduced. [0003] The estrous symptoms of sows are hereditary to a certain extent. The study found that the estrus symptoms of foreign commercial sows were not as obvious as those of local sows in my country. The obvious difference in estrus symptoms among breeds...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 周波李会智
Owner 博瑞迪生物技术(海南省)有限公司
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