A method for cultivating root-knot nematode borealis using peanut hairy roots

Abstract

The invention discloses a method for cultivating meloidogyne hapla by adopting peanut rooting. The method comprises the following steps that number 45 seeds are selected, flowered and seeded on an MSB5 culture medium for cultivation; agrobacterium rhizogenes strains A4 are taken for cultivation and activation to achieve a logarithmic growth period; the agrobacterium rhizogenes strains A4 are adopted for infecting the sterile culture 6d flowered number 45 peanut cotyledon for inducing hairy roots; peanut roots with multiple insect galls are selected to be immersed into a NaCLO solution and broken to form a mixed solution; the mixed solution is poured onto combination sieves, wherein the combination sieves are of the types of 40 meshes, 200 meshes, 325 meshes and 500 meshes in sequence from top to bottom, and sterile water is used for spraying and washing, so that the meloidogyne hapla is concentrated to one side; then, a small amount of sterile water is used for washing oversize products from the back faces of the sieves, and liquid is collected into a container to obtain meloidogyne hapla suspension; the meloidogyne hapla suspension is evenly inoculated on a peanut hairy root culture dish, and cultivation is performed to obtain a large number of eggs and second-stage larvae. The method solves the problem that a large amount of purified meloidogyne hapla cannot be obtained easily, and the reproduction rate is high.

Description

technical field [0001] The invention relates to a method for cultivating root-knot nematode, in particular to a method for cultivating root-knot nematode northern by using peanut hairy roots. Background technique [0002] Peanut is one of the most widely planted economic crops in the world, and it is the second largest edible vegetable oil and protein resource in my country. In northern my country, root-knot nematodes are mainly harmful to peanut production, especially Shandong, a major peanut province, has the largest incidence area and the most serious losses. The damaged peanuts generally have a reduction in yield of about 20%-30%, and in severe cases it can reach 70%-80%, or even no harvest (Xu Xiujuan, 2009). Peanut nematode disease is an important disease in peanut production. So far, the occurrence and damage of nematode disease cannot be well controlled. [0003] After the root-knot nematode infects peanuts, it stimulates the production of giant cells, which leads ...

AI Technical Summary

Problems solved by technology

Using the hairy root system to detect plant resistance to nematodes has been used in crops such as sugar beet, soybean, potato, tomato and sweet potato, but no one has tried to use the peanut hairy root system to cultivate root-knot nematode.