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Penicitrinine A derived from Penicillium citrinum and its application in the preparation of anti-human breast cancer drugs

A technology of human breast cancer and human breast cancer cells, applied in drug combination, antineoplastic drugs, organic chemistry, etc.

Inactive Publication Date: 2017-04-26
福建省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-human breast cancer activity, and there is no report on the proliferation inhibitory activity of this compound on human breast cancer cells SKBR-3 and MCF-7, so there is no relevant drug in the market. drug

Method used

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  • Penicitrinine A derived from Penicillium citrinum and its application in the preparation of anti-human breast cancer drugs
  • Penicitrinine A derived from Penicillium citrinum and its application in the preparation of anti-human breast cancer drugs
  • Penicitrinine A derived from Penicillium citrinum and its application in the preparation of anti-human breast cancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Fermentative production and separation and purification of the compound of embodiment 1

[0020] 1 Fermentation production

[0021] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium citrinum ( Penicillium citrinum ) IBPT-5 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number: CCTCC NO: M 2013713) was inoculated into PDA solid slant medium and cultured at 28°C 4 days in the box.

[0022] Get the Penicillium citrinum ( Penicillium citrinum ) IBPT-5 was inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl 30.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermentation broth were obtained.

[0023] 2 Obtaining the extr...

Embodiment 2

[0030] Example 2 Test of anti-tumor activity in vitro

[0031] 1 Experimental samples and experimental methods

[0032] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Precisely weigh an appropriate amount of sample, and prepare a solution with the required concentration with DMSO for activity measurement.

[0033] The subculture of cell lines and cells adopts tumor cell lines, and the tumor cells are treated with RPMI 1640 medium containing 10% FBS at 37°C in 5% CO 2 Subculture in an incubator.

[0034] Cell Proliferation Inhibitory Activity Test Method

[0035] WST-1 method Take tumor cells in the logarithmic growth phase, adjust the cell density to 5×10 per ml 4 Cells were inoculated into 96-well cell culture plates at 100 μL per well, and placed in 5% CO at 37°C. 2 cultured overnight in an incubator. Aspirate the supernatant, add 100 μL of sample-containing cu...

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Abstract

The invention relates to a penicitrinine A originated from penicillium citrinum and an application thereof in preparation of anti human breast cancer drugs. The compound has the effect of inhibiting human breast cancer cell proliferation and has the structural formula shown in the description. A fermented product is obtained through fermentation cultivation of penicillium citrinum IBPT-5, and then the compound is obtained through separating and purifying the fermented product. Experiments confirm that the compound has relatively good anti-tumor activity on human breast cancer cells SKBR-3 and MCF-7, and can be used as the human breast cancer cell proliferation inhibition drugs or antitumor drugs for research on human breast cancer.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a penicitrinine A derived from Penicillium citrinum and its application in the preparation of anti-human breast cancer drugs. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/20C12P17/18A61K31/407A61P35/00
CPCC07D491/20C12P17/18
Inventor 郑秋红刘沁颖陈立应敏刚周彤
Owner 福建省肿瘤医院