Anticoagulation blackberry seed effective component and extraction and separation method and application thereof

A technology of active ingredients and separation methods, applied in the field of anticoagulant blackberry seed active ingredients and their extraction and separation, can solve tissue ischemia, necrosis and other problems

Active Publication Date: 2015-11-04
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once blocked, tissue ischemia and necrosis will occur, resulting in serious consequences

Method used

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  • Anticoagulation blackberry seed effective component and extraction and separation method and application thereof
  • Anticoagulation blackberry seed effective component and extraction and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Blackberry seeds were purchased from the blackberry planting base in Fengqiu County, Henan Province. Rubus spp. Blackberry seeds.

[0023] The ethanol concentration in this article is the volume concentration.

[0024] Extraction and separation methods of active compounds:

[0025] (1) After the blackberry seeds are crushed, they are first extracted with petroleum ether for 4 times, each time for 3 days, after evaporating the petroleum ether, add 70% ethanol for 3 times, each time for 3 days, the extracts are combined, filtered and concentrated Obtain the total extract of ethanol, sequentially extract with petroleum ether, ethyl acetate, n-butanol, reclaim the solvent, obtain the petroleum ether part, ethyl acetate part and n-butanol part;

[0026] (2) The ethyl acetate part was subjected to 200-300 mesh silica gel column chromatography, dichloromethane-methanol gradient elution (volume ratio 100:0-1:1), combined with TLC thin-layer chromatography to obtain 6 comp...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to an anticoagulation blackberry seed effective component and an extraction and separation method and application thereof. Six compounds are obtained from the ethyl acetate portion and the n-butanol portion of blackberry seeds through a special method in a separation mode, the in-vitro anticoagulation effect of the six compounds is investigated, the result shows that the compounds from the first to the sixth all have a good anticoagulation effect, and the six compounds can be independently used or combined to serve as anticoagulants.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to an anticoagulant active ingredient of blackberry seeds, an extraction and separation method and application thereof. Background technique [0002] blackberries Rubus spp.Blackberry is an aggregate fruit plant of the genus Rubus in the Rosaceae family. It is not used for medicine and is an emerging 4 One of the small fruit trees. Native to North America, its sweet and sour fruit has high nutritional value and medicinal value. In addition to fresh food, most of it is made into quick-frozen fruit, fruit juice, fruit wine and jam. Literature search found that the research on blackberry mainly focused on the volatile oil of the fruit, the fatty acid composition of blackberry seed oil, and the flavonoids, anthocyanins and vitamins in blackberry leaves and seeds. E content analysis. [0003] Blackberry seeds are by-products of processed products such as bl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07C233/09C07C231/24C07D311/62A61P7/02
CPCC07C231/24C07C233/09C07D311/62C07J63/008
Inventor 康文艺张伟张勇谢平耀何楠余琦
Owner HENAN UNIVERSITY
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