A molecular recognition method for high-efficiency grafted camellia root and ear combination of old Camellia oleifera trees

A technology for molecular identification and camellia oleifera tree, applied in the biological field, can solve the problems of no molecular identification technology of camellia oleifera grafted stock and ear combination, no inspiration of stock and ear combination, etc., and achieves easy popularization and expansion, reduction of high disease incidence, Simple to use effects

Active Publication Date: 2018-03-16
DALIAN NATIONALITIES UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, in the field of Camellia oleifera grafting, there is only one invention patent called Camellia oleifera grafting incision (wound) treatment method (publication number is CN103109682A), and there is no enlightenment on the combination of stock and ear; and, in the prior art There is also no relevant report on the molecular recognition technology of rootstock and ear combination of Camellia oleifera grafted

Method used

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  • A molecular recognition method for high-efficiency grafted camellia root and ear combination of old Camellia oleifera trees
  • A molecular recognition method for high-efficiency grafted camellia root and ear combination of old Camellia oleifera trees
  • A molecular recognition method for high-efficiency grafted camellia root and ear combination of old Camellia oleifera trees

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Embodiment 1

[0025] (1) Using 7 kinds of clones of old Camellia oleifera and 48 kinds of camellias as materials, the DNA was extracted respectively by using the improved CTAB method. The steps are as follows:

[0026] 1) Put 10 grams of leaves of old camellia oleifera tree or camellia flower into a mortar, pour in an appropriate amount of liquid nitrogen for grinding, transfer to a centrifuge tube pre-added with 700 μL 2×CTAB, place in a water bath at 65°C for 45-60 minutes, and centrifuge ( 4°C, 1000rpm, 10min) to obtain supernatant I;

[0027] 2) Take 600 μL of the supernatant I in the centrifuge tube in step 1) and put it into a new centrifuge tube, add a mixture of phenol, chloroform and isoamyl alcohol (volume ratio 25:24:1) with a total volume of 600 μL, shake well and centrifuge (4°C, 1000rpm, 10min) to obtain supernatant II;

[0028] 3) Take 550 μL of the supernatant II into a new centrifuge tube, add 500 μL of 10×CTAB, shake well, put it in a 65°C water bath to dissolve for 2-3 m...

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Abstract

The invention discloses a molecular recognition method for rootstock-scion combination and selection in efficient old tea-oil tree and camellia grafting. According to the method, old tea-oil trees serve as rootstocks, camellia serves as scions, and the molecular recognition method of efficient grafting rootstock-scion combination and selection is obtained in the following steps that 1, the DNA of the old tea-oil trees and the DNA of the camellia are extracted; 2, a proper SSR labeling primer is screened; 3, the Dice heredity coefficients between different varieties of old tea-oil trees and different varieties of camellia are detected; 4, a rootstock-scion combination is designed according to the Dice heredity coefficients; 5, according to the rootstock-scion combination, the camellia is grafted through the old tea-oil trees. The rootstock-scion combination, with the Dice heredity coefficient range being 0.551-0.600 and 0.751-0.800, obtained through the molecular recognition method is good in grafting compatibility, high grafting disease morbidity is low, the grafting survival rate is as high as over 84%, the high grafting disease morbidity is lower than 4%, and the rootstock-scion combination can be conveniently applied and popularized in market.

Description

technical field [0001] The invention relates to a plant grafting method for selecting and matching rootstock and ear combinations, in particular to a method for selecting and matching stock and ear combinations of old camellia trees for efficient grafting of camellias by using genetic coefficients, and belongs to the field of biotechnology. Background technique [0002] Camellia oleifera, oil palm, olive and coconut are known as the four major woody edible oil plants in the world. Its main product, camellia oil, is a high-grade edible oil with clear color, fragrant taste, storage resistance and reasonable nutritional ratio, known as "Oriental olive oil". . There are a large number of low-yielding camellia oleifera forests in my country's camellia oleifera producing areas, some of which have low yields and poor benefits, and are not valuable as oleifera-producing trees. How to make full use of their remaining value to help farmers in camellia oleifera producing areas get rid ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895A01G2/30
CPCA01G2/30C12Q1/6895
Inventor 阮成江
Owner DALIAN NATIONALITIES UNIVERSITY
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