Method for rapid determination of content of short-chain fatty acids in intestinal tract or excrement

A technology for medium and short-chain fatty acids and short-chain fatty acids, which is applied in the field of short-chain fatty acid content determination, can solve the problem of not finding short-chain fatty acids, and achieve the effects of high recovery, reduced measurement time, and rapid and accurate analysis methods.

Inactive Publication Date: 2015-11-04
NANCHANG UNIV
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  • Method for rapid determination of content of short-chain fatty acids in intestinal tract or excrement
  • Method for rapid determination of content of short-chain fatty acids in intestinal tract or excrement
  • Method for rapid determination of content of short-chain fatty acids in intestinal tract or excrement

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Embodiment 1

[0029] (1) Human feces were homogeneously diluted at 1:9, and after mixing, the mixture was centrifuged for 15 min (6000 g ), and 0.5 ml supernatant was used to determine the content of short-chain fatty acids.

[0030] (2) Chromatographic analysis was carried out by Agilent 6890 N gas chromatography and HP-INNOWAX chromatographic column.

[0031] Gas phase analysis conditions: FID detector, carrier gas is N 2 ; N 2 The flow rate is 19.0 ml / min, and the split ratio is 1:10. The flow rate of air is 300 ml / min, H 2 The flow rate is 30 ml / min; the detector temperature is 240°C, and the inlet temperature is 240°C.

[0032] The heating program was as follows: after holding at 100°C for 0.5 min, the temperature was raised to 150°C at a rate of 4°C / min, and the measurement time was 13 min. The injection volume of the sample was 0.3 μl, and each sample was repeated three times independently, and the data analysis was performed by HP Chem workstation software (A.09.xx, Agilent). ...

Embodiment 2

[0036](1) Dilute the contents of the mouse colon at a ratio of 1:10, and after mixing, centrifuge the mixture for 20 min (6000 g ), and 0.5 ml supernatant was used to determine the content of short-chain fatty acids.

[0037] (2) Chromatographic analysis was carried out by Agilent 6890 N gas chromatography and HP-INNOWAX chromatographic column.

[0038] Gas phase analysis conditions: FID detector, carrier gas is N 2 ; N 2 The flow rate is 19.0 ml / min, and the split ratio is 1:10. The flow rate of air is 300 ml / min, H 2 The flow rate is 30 ml / min; the detector temperature is 240°C, and the inlet temperature is 240°C.

[0039] The heating program was as follows: after holding at 100°C for 0.5 min, the temperature was raised to 150°C at a rate of 4°C / min, and the measurement time was 13 min. The injection volume of the sample was 0.2 μl, and each sample was repeated three times independently, and the data analysis was performed by HP Chem workstation software (A.09.xx, Agile...

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Abstract

A method for rapid determination of content of short-chain fatty acids in intestinal tract or excrement comprises the following steps: (1) carrying out homogeneous dilution by using intestinal contents or distilled water for excrement at the ratio of 1:9-1:14, uniformly mixing, centrifuging a mixture for 15-20 min, and taking 0.5-1 ml of a supernatant; (2) carrying out chromatographic analysis through Agilent 6890 N gas chromatography and an HP-INNOWAX chromatographic column; gas phase analysis conditions: an FID detector is used and carrier gas is N2; N2 flow velocity is 19.0ml/min, and split ratio is 1:10; air velocity is 300 ml/min, and H2 flow velocity is 30 ml/min; detector temperature is 240 DEG C, and injection port temperature is 240 DEG C; temperature programming: after maintaining 100 DEG C for 0.5 min, heating to 150 DEG C at the temperature rising speed of 4 DEG C/min, and measuring for 13 min; inlet sample quantity is 0.2-0.5 microliter, 3 times of separate repeated measurements are carried out for each sample for each time, and data analysis is carried out by HP Chem workstation software. Only after homogenizing a sample in distilled water and centrifuging the sample, a supernatant is taken to directly carry out gas phase sample-introduction and analysis, and measurement time can be reduced remarkably. In addition, the method is simple, rapid and accurate.

Description

technical field [0001] The invention relates to the determination of short-chain fatty acid content in intestinal tract or feces. Background technique [0002] Intestinal microbial flora mainly refers to the general term of intestinal flora that exists in abundance and diversity in the distal intestinal tract of the human body and plays a key role in human health and nutrition. Gut microbes are important "microbial organs" of the human body, and are closely related to many physiological functions such as immunity, nutrition, and metabolism. The human intestinal tract provides a good habitat for microorganisms. In the adult intestinal tract, the number of microorganisms is as high as 10 14 , close to 10 times the number of human somatic cells; the mass reaches 1.2 kg, which is close to the mass of human liver; the number of genes contained in it is about 100 times that of the human body itself, and it has metabolic functions that the human body does not possess. As the larg...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 聂少平胡婕伦谢明勇
Owner NANCHANG UNIV
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