Mulberry enzyme health beverage production method

A health-care beverage and mulberry enzyme technology, applied in the field of bioengineering, can solve the problems of long fermentation time, pollution, and the inability to guarantee the absence of pathogenic bacteria, and achieve the effects of reducing loss, retaining enzyme activity and maintaining stable quality.

Inactive Publication Date: 2015-11-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a preparation method that has good taste and can keep the nutrition and health care components of mulberry enzyme to the greatest extent, and solves the problem that in the existing enzyme production process, no pathogenic bacteria pollution can be guaranteed, the fermentation time is too long, and the enzyme activity cannot be effectively prolonged. time retention issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for fermenting mulberry enzymes, comprising the following steps:

[0024] (1) Select mature mulberry with fresh color and high natural enzyme content as the main raw material for fermentation.

[0025] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 30% of the volume, add 10% of the total liquid volume % of the raw honey is for the fermentation stock solution.

[0026] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;

[0027] (4) According to the inoculation ratio of 5%, the nectar-derived bacteria and lactobacillus were respectively taken and inserted into the fermentation stock solution, and fermented for 7 days, with a pH value of 7.0 and a temperature of 25°C.

[0028] (5) Coarse filtration is carried out first...

Embodiment 2

[0030] A method for fermenting mulberry enzymes, comprising the following steps:

[0031] (1) Select mature mulberry with fresh color and high natural enzyme content as the main raw material for fermentation.

[0032] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 40% of the volume, add 15% of the total liquid volume % of the raw honey is for the fermentation stock solution.

[0033] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;

[0034] (4) According to the inoculation ratio of 4%, the nectar-derived bacteria and lactic acid bacteria were respectively taken and inserted into the fermentation stock solution, and fermented for 10 days, with a pH value of 6.5 and a temperature of 28°C.

[0035] (5) Coarse filtration is carried o...

Embodiment 3

[0037] A method for fermenting mulberry enzymes, comprising the following steps:

[0038] (1) Select mature mulberry with fresh color and high natural enzyme content as the main raw material for fermentation.

[0039] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 50% of the volume, add 20% of the total liquid volume % of the raw honey is for the fermentation stock solution.

[0040] (3) Prepare LB medium, activate the original preserved strain, and cultivate the fermented bacterial liquid obtained for about 48 hours for later use; (4) According to the inoculation ratio of 3%, the nectar-derived bacteria, Lactobacillus casei and Lactobacillus plantarum were respectively taken and inserted The fermentation stock solution was fermented for 14 days, with a pH value of 7.0 and a temperature of 25°C.

[0041] (5) Coarse filtration is carried out fi...

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Abstract

The present invention provides a method for preparing a health beverage through mulberry fermentation. The process characteristics are that mulberry is adopted as a main raw material, Bacillus amyloliquefaciens H47 preserved in China Center for Type Culture Collection on June 29, 2013 and having the preservation number of CCTCC M 2013283 and lactic acid bacteria are adopted as fermentation strains, mulberry, raw honey and purified water added according to a volume ratio of the mulberry to the raw honey to the purified water of 2-5:1-3:7-2, uniform mixing is performed to prepare a mulberry fermentation raw liquid, a honey source bacterial and lactic acid bacterial seed liquid is inoculated into the mulberry fermentation raw liquid according to a ratio of 1-5%, fermentation is performed for 6-14 d at a temperature of 25-35 DEG C under the pH value of 5.0-8.0 to obtain a mulberry enzyme beverage crude product, coarse filtration is performed with a 20-32 mesh filtration sieve to primarily filter out large particle residue, and fine filtration is performed with a 80-100 mesh filtration sieve to further filter out small particle residue to make the liquid present the clear state so as to obtain the mulberry enzyme health beverage finished product.

Description

technical field [0001] The invention relates to a production method of fruit enzymes, in particular to a fermentation method of fresh mulberry fruit enzyme health drinks, and belongs to the technical field of bioengineering. Background technique [0002] Enzyme is a Japanese name for enzyme (enzyme), and later the initial biologically active products obtained by microbial fermentation are collectively referred to as enzymes. However, because many producers pay more attention to adding different fruits, but do not pay attention to the use of fermenting microorganisms and wild functional materials, the development and application of enzyme products are affected by their side effects and poor functionality. At present, some studies believe that fruit enzymes claim to have three major functions: detoxification, beauty, and nourishing qi and blood; it is also clear that there is amylase in the mouth, pepsin in the stomach, lipase is secreted by the liver, and the spleen is also c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/02A23L2/84A23L2/72
CPCA23L2/382A23L2/72A23V2002/00
Inventor 廖祥儒张永张言周田乔鹏蔡宇杰管政兵
Owner JIANGNAN UNIV
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