Compound platelet gel and preparation method thereof

Abstract

The invention discloses compound platelet gel and a preparation method thereof. The preparation method includes: taking chitosan which is a natural material high in biodegradability and biocompatibility as a biological scaffold and a drug delivery carrier; activating fibrinogen in PRP to be a fibrin netty structure to be connected to chitosan molecules to form a gel carrier scaffold; and enabling platelet-derived cytokines to be adhered and anchored in the gel carrier scaffold to form the compound platelet gel. The compound platelet gel has multiple functions of quickly promoting tissue regeneration, bleeding stopping, bacterium resisting, pain relieving and humidity maintaining, and is simple to prepare, convenient to use, capable of realizing slow release of platelet-derived growth factors and active substances, lasting in biological effect and suitable for application in restorative treatment of soft or hard tissue defect, skin or mucosa refractory damage or ulcer, articular cartilage degeneration and ischemic tissue necrosis.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a composite platelet gel and a preparation method thereof. Background technique [0002] Recent studies have found that, in addition to performing classic physiological functions such as coagulation and hemostasis, platelets also store a large number of important cytokines and biologically active substances synthesized by the human body, especially active substances closely related to tissue regeneration and wound repair, endogenous Cannabinoid-like analgesic substances, and broad-spectrum antimicrobial peptides (such as natural defensins, etc.). When the body tissue is damaged, platelets are the first to be quickly recruited to the wound site, and after activation, the natural cytokines (mainly including platelet-derived growth factor, vascular endothelial cell growth factor, and Factors, insulin-like growth factor-1, basic fibroblast growth factor and hepatocy...

AI Technical Summary

Problems solved by technology

The inventors of the present application also carried out a series of in vivo and in vitro studies on PRP promoting angiogenesis, wound healing, hematopoietic stem cell proliferation, and liver regeneration in the early stage, and found that: PRP does have the effect of promoting tissue regeneration, wound healing, and inhibiting scar formation. Comparable advantages; however, it is also found that PRP has the following serious defects in use, which limits its wide application in clinical practice:

[0003] 1. When PRP is applied, it is firstly activated by thrombin and a solution containing calcium ions, and the activated platelets release cytokines to play a role; The effect time is too short, and the biological effect cannot last;

[0004] 2. In the process of PRP activation, the rapidly formed fibrin network has poor elasticity and stability, and is easy to shrink, which is not conducive to the chemotaxis and implantation of stem / progenitor cells in vivo;

[0005] 3. PRP is poorly combined with conventionally used surgical dressings, resulting in the loss of most active ingredients;

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