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Method for separating and purifying kandelia candel leaf total flavonoids

A technology for separation and purification of total flavonoids, which is applied in the field of separation and purification of total flavonoids from candela leaves, can solve the problems of low purity of total flavonoids and affect the efficacy of drug development, etc., and achieve the effect of strong whitening and freckle removing activity, simple method and strong pertinence

Inactive Publication Date: 2015-11-11
CHENGDU YICHUANGSI BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above two purification methods are exemplified. The main disadvantages of the above two methods are: the obtained total flavonoids have low purity. After passing through the macroporous resin, the purity is less than 20%, even after using a large amount of toxic organic solvents. The total flavonoid content does not exceed 30%, which affects the efficacy of drug development, and the use of toxic organic solvents for extraction can easily lead to hidden dangers of environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment provides a method for separation and purification of total flavonoids from leaves of candelabra, which specifically includes the following steps:

[0030] (1) Extraction: take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 65% as the extraction agent, the ratio of solid to liquid is 1:15 (mass to volume ratio), the extraction temperature is 80°C, and the extraction time is 1.0h. Extract 2 times;

[0031] (2) Separation and purification: Purify the total flavonoids of candela leaves obtained in step (1) with HPD722 macroporous adsorption resin. The specific purification process is: the concentration of total flavonoids in the sample is 5.0mg / ml, and the flow rate of the sample is 3BV / h. The loading volume is 6.5BV resin column volume, the adsorption time is 30min, wash with 3.5 times water to remove impurities, the eluent is ethanol with a volume fraction of 50%, the elution flow rate is 3BV / h, and the ...

Embodiment 2

[0033] This embodiment provides a method for separation and purification of total flavonoids from leaves of candelabra, which specifically includes the following steps:

[0034] (1) Extraction: take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 70% as the extractant, the ratio of solid to liquid is 1:15, the extraction temperature is 60°C, the extraction time is 1.5h, and the extraction is performed twice;

[0035] (2) Separation and purification: Purify the total flavonoids of candela leaves obtained in step (1) with HPD722 macroporous adsorption resin. The specific purification process is: the concentration of total flavonoids in the sample is 5.0mg / mL, and the flow rate of the sample is 2.5BV / h , the loading volume is 6BV resin column volume, the adsorption time is 30min, wash with 3.5 times water to remove impurities, the eluent is ethanol with a volume fraction of 35%, the elution flow rate is 4BV / h, and the eluent dosa...

Embodiment 3

[0037] This embodiment provides a method for separation and purification of total flavonoids from leaves of candelabra, which specifically includes the following steps:

[0038] (1) Extraction: take candela leaves and crush them into coarse powder, take 1000g, use ethanol with a volume fraction of 70% as the extraction agent, the ratio of solid to liquid is 1:15, the ratio of solid to liquid is the ratio of mass to volume, the extraction temperature is 80°C, and the extraction time is 2.0h, extract 2 times;

[0039] (2) Separation and purification: Purify the total flavonoids of candela leaves obtained in step (1) with HPD100 macroporous resin. The loading volume is 5.5BV resin column volume, the adsorption time is 30min, wash with 3.5 times water to remove impurities, the eluent is ethanol with a volume fraction of 50%, the elution flow rate is 3BV / h, and the eluent dosage is 4BV resin column volume , collected the ethanol liquid, recovered the solvent under reduced pressure...

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Abstract

The invention discloses a method for separating and purifying kandelia candel leaf total flavonoids. The method comprises the steps that 1, kandelia candel leaves are taken and smashed into coarse powder, extraction is conducted on the coarse powder for two times through a 65%-75% ethanol solution which accounts for 15 times of the coarse powder, the extraction time is 1.0-2.0 h each time, and extracting solutions are mixed; 2, concentrating is conducted on the extracting solution obtained in the first step to make the concentration of the extracting solution range from 4 mg / ml to 5 mg / ml, centrifugation is conducted, supernatant liquid is taken and purified through nonpolar macroporous absorption resin, absorption is conducted for 30 min, water washing is conducted for removing impurities, and elution is conducted through 40%-70% ethyl alcohol; 3, eluant obtained in the second step is collected, decompression recycling of the solvent is conducted, drying and smashing are conducted, and a final product is obtained. The content of the kandelia candel leaf total flavonoids is at least 60%. The separation and purification method of the kandelia candel leaves is simple, convenient, efficient, environmentally friendly and strong in pertinence, and the kandelia candel leaf total flavonoids which are high in purity and whitening and freckle-removing activity can be obtained.

Description

technical field [0001] The invention belongs to the technical field of bioseparation, and specifically refers to a method for separating and purifying total flavonoids from candela leaves. Background technique [0002] Candelia candel (Kandelia candel) is a plant belonging to the mangrove family Candelaceae. It is the most cold-resistant tree species among mangrove plants in my country. From south to north in the distribution area of ​​mangrove plants in China, the species of mangrove plants gradually decrease. North end 28o25'N. The chemical components in candela mainly include flavonoids, tannins, sterols, fatty acids and polysaccharides. In recent years, many scholars have studied various chemical components in candela, and found some functions with drug development value. For example, scholar Li Baocai reported in the article "Research on Lipid Chemistry and Active Substances of Mangrove Candelan Leaves": total flavonoids of candela leaves , the content is 29.3%, has an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185
Inventor 周洁
Owner CHENGDU YICHUANGSI BIOLOGICAL SCI & TECH