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Compound microbial remediation agent for lead and zinc contaminated soil and preparation method of compound microbial remediation agent

A technology for compound microorganisms and polluted soil, applied in the field of lead-zinc contaminated soil compound microbial remediation agent and its preparation, can solve the problems of soil desertification, soil degradation, affecting soil quality, etc., achieving no chemical residues and secondary pollution, and good remediation. effect of effect

Active Publication Date: 2015-11-11
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to natural subsidence and rain subsidence, heavy metals will cause soil sandification and crystallization, which will seriously affect soil quality and cause a lot of soil degradation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] The preparation method of the lead-zinc polluted soil composite microbial remediation agent that the present invention relates to comprises the following steps:

[0025] Step 1: Fermentation:

[0026] Adjust the pH of the liquid fermentation medium A to 6.0-6.5, and carry out conventional sterilization treatment, insert Micrococcus flavum PS5 according to the aseptic operation of 6-9% of the liquid mass, keep the constant temperature at 35±1°C, and stir at a speed of 200-250rpm. Ventilation 0.27-0.30M 3 / min, tank pressure 0.07-0.08MPa, continuous fermentation for 72-76hr, the number of cells in the fermented liquid reached 2.0×10 by microscope detection and counting 9 Individual / mL to stop the fermentation;

[0027] Adjust the pH of the liquid fermentation medium B to 6.0-6.5, and carry out conventional sterilization treatment, insert Pseudopallidalus saccharolyticum ZS2 aseptically according to the liquid weight 6-9%, constant temperature 35±1°C, stirring speed 200 ...

Embodiment 1

[0039] Step 1: Fermentation:

[0040] Adjust the pH of the liquid fermentation medium A to 6.0, and carry out conventional sterilization treatment, insert Micrococcus flavum PS5 according to the aseptic operation of 9% liquid mass, keep the constant temperature at 34°C, stir at 250rpm, and ventilate at 0.27M 3 / min, tank pressure 0.08MPa, continuous fermentation for 72hrs, and the number of cells in the fermentation liquid reached 2.0×10 by microscope detection and counting 9 Individual / mL to stop the fermentation;

[0041] Adjust the pH of the liquid fermentation medium B to 6.5, and carry out conventional sterilization treatment, insert Pseudopallidum saccharolyticum ZS2 aseptically according to the liquid weight 6%, constant temperature 36°C, stirring speed 200rpm, ventilation volume 0.32M 3 / min, tank pressure 0.07MPa, continuous fermentation for 76hrs, and the number of cells in the fermentation liquid reached 2.0×10 9 cells / mL to stop the fermentation.

[0042] The fo...

Embodiment 2

[0051] Step 1: Fermentation:

[0052] Adjust the pH of the liquid fermentation medium A to 6.2, and carry out conventional sterilization treatment, insert Micrococcus flavum PS5 according to the aseptic operation of 7% liquid mass, constant temperature 35°C, stirring speed 220rpm, ventilation volume 0.28M 3 / min, tank pressure 0.07MPa, continuous fermentation for 74hrs, and the number of cells in the fermentation liquid reached 2.0×10 9 Individual / mL to stop the fermentation;

[0053] Adjust the pH of the liquid fermentation medium B to 6.2, and carry out conventional sterilization treatment, insert Pseudopallidum saccharolyticum ZS2 aseptically according to the liquid weight 7%, constant temperature 35°C, stirring speed 220rpm, ventilation volume 0.30M 3 / min, tank pressure 0.07MPa, continuous fermentation for 74hrs, and the number of cells in the fermentation liquid reached 2.0×10 9 cells / mL to stop the fermentation.

[0054] The formula of liquid fermentation medium A is...

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Abstract

The invention relates to a compound microbial remediation agent for lead and zinc contaminated soil and a preparation method of the compound microbial remediation agent. Heavy metal in tailings generated during lead and zinc mineral exploration and dumped ore diffuse towards the surrounding regions under the supergene geochemical process including rainfall, weathering and the like, so as to cause strong impact on the structure and the function of an ecological system. According to the invention, the preparation method of the compound microbial remediation agent comprises the following steps: culturing micrococcus flavus PS5 and pseudochrobactrum asaccharolytica ZS2 by a special liquid fermentation medium respectively, collecting the fermentation broth, carrying out centrifugal separation to remove water in the fermentation broth, and sufficiently stirring the two kinds of viscous thalli obtained by centrifugal separation for uniform mixing so as to obtain light-yellow viscous thallus cells; adding glycerinum into the obtained light-yellow viscous thallus cells and stirring for uniform mixing, and then adding kieselguhr into the obtained light-yellow viscous thallus cells and stirring for uniform mixing, thus obtaining a light-yellow solid preparation which is the target product. According to the invention, lead and zinc ions are enriched and passivated in the cells through biological metabolism of the microbes, and protide organisms generated are combined for adsorbing the metal ions, so that the degradation efficiency is increased, the compound microbial remediation agent has good environment adapting capacity, for example, to the temperature and the pH value, and no chemical residue or secondary pollution is generated after remediation.

Description

technical field [0001] The invention belongs to the technical field of soil bioremediation, and in particular relates to a compound microbial remediation agent for lead-zinc polluted soil and a preparation method thereof. Background technique [0002] The heavy metals in the tailings and abandoned ores of lead-zinc mining spread to surrounding areas through supergene geochemical processes such as rainfall and weathering, which have a huge impact on the structure and function of the ecosystem. Due to natural settlement and rain deposition, heavy metals will cause soil sandification and crystallization, which will seriously affect soil quality and cause a large amount of soil degradation. At present, the remediation of soil pollution in lead-zinc mining areas at home and abroad mainly includes physical, chemical and biological remediation technologies, among which physical and chemical remediation may cause secondary pollution. However, bioremediation has no secondary polluti...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10
Inventor 沈卫荣孙晓宇陈锐瞿佳赵玲侠韩丽萍路鹏鹏门欣
Owner MICROBIOLOGY INST OF SHAANXI