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Cadmium-IgM chelate, and preparation method and application thereof

A chelate, chelation reaction technology, applied in the field of detection, to achieve the effect of improving repeatability and accuracy

Inactive Publication Date: 2015-11-11
广州锦海骢健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For the serious problem of cadmium pollution, the object of the present invention is to provide a kind of cadmium-IgM chelate and preparation method thereof, and establish the qualitative and quantitative detection method of cadmium-IgM chelate, so that quantitative detection cadmium-IgM chelate Application in evaluating the degree of cadmium pollution in an area

Method used

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  • Cadmium-IgM chelate, and preparation method and application thereof
  • Cadmium-IgM chelate, and preparation method and application thereof
  • Cadmium-IgM chelate, and preparation method and application thereof

Examples

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preparation example Construction

[0060] The present invention also provides a kind of preparation method of cadmium-IgM chelate, comprises the following steps:

[0061] A) chelation reaction of cadmium and IgM: adding cadmium ions to human IgM or IgM recombined according to biological methods for chelation reaction to obtain a reaction solution;

[0062] B) Extraction of purified cadmium-IgM chelate: Immunoaffinity chromatography is used to remove unreacted IgM and excess cadmium ions in the reaction solution to obtain cadmium-IgM chelate. The specific steps are as follows:

[0063] (1) Dissolving sample: add physiological saline to the reaction solution obtained through step A), the cadmium-IgM chelate is redissolved

[0064] (2) Equilibrium chromatography column: use the dilution buffer to flush the pipeline of the chromatography column, fill the chromatography column with a filler that can specifically bind to IgM, and continue to use the dilution buffer to balance the chromatography column after loading t...

Embodiment 1

[0158] Example 1 : the preparation method of cadmium-IgM chelate, comprises the following steps:

[0159] A) the chelation reaction of cadmium and IgM: add cadmium ion to carry out chelation reaction in the IgM of human source, obtain reaction solution;

[0160] Reagent preparation:

[0161] 1) Borate buffer (0.01M): Weigh 0.31g of boric acid and dissolve it in 400ml of ultrapure water, adjust the pH to 9.0 with 0.1M NaOH, and dilute to 500mL.

[0162] 2) IgM solution: Weigh 4.0mg IgM and dissolve in 4.0mL0.01MpH9.0 borate buffer, fully shake and dissolve, and prepare a 1.0mg / mL protein solution;

[0163] 3) 5mmol / LEDTA+200mmol / LNaHCO 3 Solution: weigh EDTA·2H 2 O1.86g, NaHCO 3 Dissolve 16.8g in 900mL ultrapure water, adjust the pH to 8.0 with 1.0M NaOH and dilute to 1000ml, autoclave, and store at room temperature;

[0164] 4) ITCBE (purchased from Japan Tongren Chemical Research Institute, item number M030)

[0165] 5) Dialysis bag (molecular weight cut off 14000) (B...

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Abstract

The invention discloses a cadmium-IgM chelate, and a preparation method and an application of the chelate. The cadmium-IgM chelate is prepared from cadmium ions and IgM through chelation of a sulfydryl group or / and a cysteine residue. A method for qualitatively quantitatively testing the cadmium-IgM chelate is established to facilitate the application of evaluating a cadmium pollution degree in one area through quantitatively testing the cadmium-IgM chelate. Through quantitatively testing the cadmium-IgM chelate in serum of people in the area, the situation that people in the area polluted by cadmium can be indirectly reflected, and the cadmium pollution degree in the area can be indirectly reflected. According to the quantitative testing method of the cadmium-IgM chelate, the accuracy and the repeatability of testing are greatly improved.

Description

technical field [0001] The invention relates to the detection field, and more specifically relates to a cadmium-IgM chelate compound, a preparation method and application thereof. Background technique [0002] IgM in serum is composed of 5 monomers connected by a J chain and disulfide bond to form a pentamer, with the largest molecular weight of 970kD and a sedimentation coefficient of 19S, called macroglobulin. In terms of molecular structure, IgM has no hinge region, and Cμ2 may replace the function of the hinge region. IgM is the earliest immunoglobulin in the process of biological evolution. In the process of individual development, whether it is Ig (SmIg) on ​​the surface of B cell membrane or Ig synthesized and secreted into serum, IgM is the first Ig to appear, and the fetus in the late embryonic development has the ability to produce IgM. In the process of antigen stimulation to induce humoral immune response, generally IgM is also produced first. IgM accounts for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/53G01N27/62G01N1/28G01N27/447G01N27/626
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 广州锦海骢健康科技有限公司
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