Method for preparing electrochemiluminescence immunosensor for alpha fetoprotein based on Au-g-C3N4 nanocomposite and application

A nanocomposite material, au-g-c3n4 technology, is applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, scientific instruments, etc., which can solve the problem of poor reproducibility, high cost and high cost of radioimmunoassay. Difficult to preserve and other problems, to achieve the effect of enhancing the intensity of electrochemiluminescence, low detection limit, and simplifying the electrode preparation process

Inactive Publication Date: 2015-11-11
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, enzyme-linked immunoassay and fluorescent immunoassay are costly, and there is a pr

Method used

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  • Method for preparing electrochemiluminescence immunosensor for alpha fetoprotein based on Au-g-C3N4 nanocomposite and application
  • Method for preparing electrochemiluminescence immunosensor for alpha fetoprotein based on Au-g-C3N4 nanocomposite and application

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Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1 A kind of based on Au-g-C 3 N 4 Preparation method of electrochemiluminescent immunosensor for alpha-fetoprotein of NHs

[0036] (1) Use 1.0μm, 0.3μm, 0.05μm Al 2 o 3Polish the GCE with a diameter of 4 mm in turn with polishing powder, ultrasonically clean them in ethanol and ultrapure water for 3 minutes, blow dry with nitrogen, and take 6 μLAu-g-C 3 N 4 The NHs dispersion was applied onto the surface of the electrode, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded Au-g-C 3 N 4 NHs get Au-g-C 3 N 4 NHs / GCEs;

[0037] (2) Take 50 μL of 1 μg / mL anti-AFP standard solution and drop-coat it on the surface of the electrode, incubate in a refrigerator at 4°C for 12 hours, wash with PBS (pH7.4) buffer solution to remove physical adsorption to obtain anti-AFP / Au-g-C 3 N 4 NHs / GCEs;

[0038] (3) Take 50 μL of BSA solution with a mass fraction of 1% and drop-coat it on the electrode surface, incuba...

Embodiment 2

[0040] Embodiment 2 A kind of based on Au-g-C 3 N 4 Preparation method of electrochemiluminescent immunosensor for alpha-fetoprotein of NHs

[0041] (1) Use 1.0μm, 0.3μm, 0.05μm Al 2 o 3 Polish the GCE electrode with a diameter of 4 mm in turn with polishing powder, ultrasonically clean it in ethanol and ultrapure water for 3 minutes, blow dry with nitrogen, and take 8 μ LAu-g-C 3 N 4 The NHs dispersion was applied onto the surface of the electrode, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded Au-g-C 3 N 4 NHs get Au-g-C 3 N 4 NHs / GCEs;

[0042] (2) Take 50 μL of 2.0 μg / mL anti-AFP standard solution and drop-coat it on the surface of the electrode, incubate in a refrigerator at 4°C for 12 hours, wash with PBS (pH7.4) buffer solution to remove physical adsorption to obtain anti-AFP / Au-g-C 3 N 4 NHs / GCEs;

[0043] (3) 50 μL of BSA solution with a mass fraction of 2% was drip-coated on the electrode surface, ...

Embodiment 3

[0045] Embodiment 3 A kind of based on Au-g-C 3 N 4 Preparation method of electrochemiluminescent immunosensor for alpha-fetoprotein of NHs

[0046] (1) Use 1.0μm, 0.3μm, 0.05μm Al 2 o 3 Polish the GCE with a diameter of 4 mm in turn with polishing powder, ultrasonically clean them in ethanol and ultrapure water for 3 min, blow dry with nitrogen, and take 10 μLAu-g-C 3 N 4 The NHs dispersion was applied onto the surface of the electrode, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded Au-g-C 3 N 4 NHs get Au-g-C 3 N 4 NHs / GCEs;

[0047] (2) Take 50 μL of 2.5 μg / mL anti-AFP standard solution and drop-coat it on the surface of the electrode, incubate in a refrigerator at 4°C for 12 hours, wash with PBS (pH7.4) buffer solution to remove physical adsorption to obtain anti-AFP / Au-g-C 3 N 4 NHs / GCEs;

[0048] (3) Take 50 μL of BSA solution with a mass fraction of 3% and drop-coat it on the electrode surface, incuba...

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Abstract

The invention relates to the technical field of electrochemiluminescence immunosensors, in particular to a method for preparing an electrochemiluminescence immunosensor for alpha fetoprotein based on an Au-g-C3N4 nanocomposite and application. A dispensing method is adopted to modify the Au-g-C3N4 nanocomposite to the surface of an electrode to serve as a luminescent material and an antibody capturing substrate, and the excellent electrical conductivity and catalytic performances of gold nanoparticles can effectively improve light-emitting signals of the sensor. Based on good specificity between antigen-antibodies, the sensor is used for detecting alpha fetoproteins, and the alpha fetoproteins are detected according to the difference of the electrochemiluminescence strength of the alpha fetoproteins with different concentrations.

Description

technical field [0001] The invention relates to the technical field of electrochemiluminescence immunosensors, in particular to a sensor based on Au-g-C 3 N 4 Preparation method and application of electrochemiluminescence immunosensor for alpha-fetoprotein of nanocomposite material. Specifically related to a g-C 3 N 4 Nanosheets (g-C 3 N 4 NSs) as a luminescent material, the preparation and application of an electrochemiluminescent immunosensor with Au as an antibody capture substrate with both electrical conductivity and catalytic function. Background technique [0002] Alpha-fetoprotein (AFP) is a marker of liver cancer. The content of AFP in normal human serum is not higher than 20 μg / L. However, when liver cells become cancerous, its content in serum will increase sharply. A simple, rapid and highly sensitive method for detecting AFP is of great significance for the early diagnosis and differentiation of liver cancer. [0003] There are many immunological methods ...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/68G01N33/531
Inventor 郑香丽周长利夏方诠田栋花小霞
Owner UNIV OF JINAN
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