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A nico-based 2 o 4 Electrochemiluminescence Immunosensor for Thermosensitized Thyroglobulin in Nanosheets

A technology of thyroglobulin and electrochemistry, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of poor conductivity and limited active sites, so as to improve specificity, accelerate diffusion speed, and improve detection sensitivity Effect

Inactive Publication Date: 2021-07-13
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pNiCo 2 o 4 NSs still face the two major challenges of poor electrical conductivity and limited active sites.

Method used

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  • A nico-based <sub>2</sub> o <sub>4</sub> Electrochemiluminescence Immunosensor for Thermosensitized Thyroglobulin in Nanosheets
  • A nico-based <sub>2</sub> o <sub>4</sub> Electrochemiluminescence Immunosensor for Thermosensitized Thyroglobulin in Nanosheets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. A NiCo-based 2 o 4 The preparation method of the thermally sensitized thyroglobulin electrochemiluminescence immune device of nanosheets is characterized in that it comprises the following steps:

[0029] (1) The glassy carbon electrode (GCE) is first mechanically polished and polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned, and finally washed with ethanol, dilute Wash thoroughly with acid and water;

[0030] (2) Add dropwise 3 uL of amino-functionalized pNiCo with a concentration of 4 mg / mL 2 o 4 The NSs / SWCNTs suspension was placed on the surface of a clean glassy carbon electrode, dried under an infrared lamp, and cooled to room temperature to prepare pNiCo 2 o 4 NSs / SWCNTs modified electrodes;

[0031] (3) Add 3 uL 1 wt.% glutaraldehyde dropwise to the electrode interface prepared in step (2), and react...

Embodiment 2

[0036] The pNiCo of Example 1 above 2 o 4 Preparation of NSs materials: Prepare 10 mM Co(NO 3 ) 2 ·6H 2 O, 5 mM Ni(NO 3 ) 2 ·6H 2 O and 22.5 mM hexamethylenetetramine mixed solution, then the mixed solution was transferred to a 500 mL polytetrafluoroethylene reactor liner, magnetically stirred at 150 °C for 12 h, cooled to room temperature, centrifuged, washed, Dry in an oven at 60 °C overnight, and finally, calcined at 350 °C in air for 2 h to obtain the final product pNiCo 2 o 4 NSs, its scanning electron microscope (SEM), transmission electron microscope (TEM) and high resolution transmission electron microscope (HRTEM) see figure 1 .

Embodiment 3

[0038] The amino-functionalized NiCo of the above-mentioned embodiment 1 2 o 4 / SWCNTs suspension was prepared by the following method: 200 μL of the pNiCo prepared in Example 2 with a concentration of 4 mg / mL 2 o 4 NSs solution and 200 μL single-walled carbon nanotubes (SWCNTs, purchased from Alfa Aesar (Tianjin) Chemical Co., Ltd.) solution with a concentration of 5 mg / mL were ultrasonically mixed, and stirred at room temperature for 12 h to obtain pNiCo 2 o 4 NSs / SWCNTs suspension; then, 100 μL of 10 mM 3-aminopropyltriethoxysilane (APTES) was added to the solution, shaken at room temperature for 6 h, centrifuged and washed to obtain amino-functionalized pNiCo 2 o 4 NSs / SWCNTs suspension and stored in a 4°C refrigerator for later use.

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Abstract

The invention discloses a NiCo-based 2 o 4 Nanosheet heat-sensitized thyroglobulin electrochemiluminescence immune device, characterized by pNiCo loaded on single-walled carbon nanotubes (SWCNTs) as the conductive carrier 2 o 4 Bifunctional catalyst pNiCo prepared from NSs 2 o 4 NSs / SWCNTs, which have excellent catalytic performance for oxygen evolution reaction (OER), increase the concentration of oxygen in the solution, thereby enhancing the luminescence intensity of luminol. And pNiCo 2 o 4 NSs / SWCNTs have excellent photothermal properties, which can effectively convert 808 nm laser light into heat, increase the electrode surface temperature, and then form a temperature gradient layer on the electrode surface, accelerate the diffusion rate of electroactive substances to the electrode surface, and further enhance the luminol glowing signal. The invention has the advantages of high sensitivity, wide linear range, strong specificity, etc., can be used for the detection of thyroglobulin (Tg), and has relatively important value and practical significance in clinical application.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and specifically relates to a NiCo-based 2 o 4 Preparation method and application of heat-sensitized thyroglobulin electrochemiluminescence immune device of nanosheets. Background technique [0002] Thyroid cancer is a relatively common malignant tumor of endocrine system origin. Epidemiological studies have shown that the incidence of thyroid cancer can reach about 345 / 100,000 people. Due to the insidious onset of thyroid cancer, there is a lack of effective early diagnosis methods in clinical practice at this stage. Although imaging examinations such as ultrasound and CT can improve the early screening level of thyroid cancer, their diagnostic sensitivity or specificity still has certain limitations. Thyroglobulin (Tg) is a sugar chain protein that is significantly released during thyroid follicular epithelial lesions. The increase in its expression...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/416
CPCG01N27/305G01N27/308G01N27/3278G01N27/416
Inventor 戴宏房丹丹李佳宁高利红张书培衣欢郑祥钦颜建英
Owner FUJIAN NORMAL UNIV
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