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A chloramphenicol molecularly imprinted electrochemiluminescent sensor and method for detecting chloramphenicol

A technology of molecular imprinting and chloramphenicol, applied in the direction of chemiluminescence/bioluminescence, electrochemical variables of materials, analysis through chemical reactions of materials, etc., can solve the problems of poor selectivity and low detection sensitivity of chloramphenicol, and achieve Strong environmental tolerance, high affinity and stability, strong controllable structure

Inactive Publication Date: 2018-02-09
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The embodiment of the invention discloses a chloramphenicol molecularly imprinted electrochemiluminescence sensor and a method for detecting chloramphenicol, which are used to solve the problems of low detection sensitivity and poor selectivity of chloramphenicol in spectrophotometry

Method used

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  • A chloramphenicol molecularly imprinted electrochemiluminescent sensor and method for detecting chloramphenicol
  • A chloramphenicol molecularly imprinted electrochemiluminescent sensor and method for detecting chloramphenicol
  • A chloramphenicol molecularly imprinted electrochemiluminescent sensor and method for detecting chloramphenicol

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Experimental program
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Embodiment 1

[0069] 1.1 Synthesis of poly-o-phenylenediamine-chloramphenicol film

[0070] First, the gold electrode was polished with 0.05 μm alumina powder, and the treated gold electrode was ultrasonically oscillated in ethanol and distilled water for 5 min, rinsed with distilled water, and dried for later use.

[0071] 0.0646g chloramphenicol and 0.0316g o-phenylenediamine are dissolved in 20mL pH and be that the amount concentration of 5.2 substance is in the acetic acid buffer solution of 0.1mol / L, obtain chloramphenicol and o-phenylenediamine maximum concentration and be 10mmol / L respectively , using the acetic acid buffer solution as the electrolyte, the Ag / AgCl electrode as the reference electrode, the platinum electrode as the counter electrode, and the gold electrode as the working electrode, using cyclic voltammetry to scan, the voltage range is 0-0.8V, and the scan rate is 0.05 V / s, the number of scanning circles was 30 circles, and a poly-o-phenylenediamine-chloramphenicol fi...

Embodiment 2

[0080] 0.323g chloramphenicol and 0.079g o-phenylenediamine are dissolved in 20mL pH 5.2 and the amount concentration of substance is in the acetic acid buffer solution of 0.05mol / L, obtains that the final concentration of chloramphenicol is 50mmol / L, and the final concentration of o-phenylenediamine The concentration is 25mmol / L, the acetic acid buffer solution is used as the electrolyte, the Ag / AgCl electrode is used as the reference electrode, the platinum electrode is used as the counter electrode, and the graphite electrode is used as the working electrode. Cyclic voltammetry is used for scanning, and the voltage range is 0-0.8V , the scanning speed was 0.05V / s, and the number of scanning circles was 40 circles, and a poly-o-phenylenediamine-chloramphenicol film was obtained on the surface of the gold electrode.

[0081] Rinse the poly-o-phenylenediamine-chloramphenicol film on the surface of the gold electrode with 0.01mol / L NaOH solution, rinse for 30min, and wash away t...

Embodiment 3

[0083] 0.162g chloramphenicol and 0.158g o-phenylenediamine are dissolved in the acetic acid buffer solution that 20mL pH is 5.2 substance concentration and are 0.2mol / L, obtain chloramphenicol final concentration and be 25mmol / L, o-phenylenediamine final concentration The concentration is 50mmol / L, the acetic acid buffer solution is used as the electrolyte, the Ag / AgCl electrode is used as the reference electrode, the platinum electrode is used as the counter electrode, and the platinum electrode is used as the working electrode. Cyclic voltammetry is used for scanning, and the voltage range is 0-0.8V , the scanning speed was 0.05V / s, and the number of scanning circles was 20 circles, and a poly-o-phenylenediamine-chloramphenicol film was obtained on the surface of the gold electrode.

[0084] Rinse the poly-o-phenylenediamine-chloramphenicol film on the surface of the gold electrode with 0.01mol / L HCl solution, rinse for 15min, and wash away the chloramphenicol in the poly-o-...

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Abstract

The embodiment of the present invention discloses a chloramphenicol molecularly imprinted electrochemiluminescent sensor, comprising an inert electrode and a chloramphenicol molecularly imprinted polymer film on the surface of the inert electrode; the chloramphenicol molecularly imprinted polymer film is prepared by the following steps: A three-electrode system was constructed with an acetic acid buffer solution containing chloramphenicol and o-phenylenediamine as the electrolyte, and an inert electrode as the working electrode; by cyclic voltammetry, the polymerization potential was 0-0.8V, and poly-o-phenylenediamine-chloro Mycin film; after elution with eluent, obtain chloramphenicol molecularly imprinted polymer film; wherein, the pH value of the acetic acid buffer solution is 5.2, and the substance concentration of the acetic acid buffer solution is 0.05~0.2mol / L; Chloramphenicol The concentration of mycin and o-phenylenediamine substances are 1-50mmol / L respectively. The chloramphenicol molecularly imprinted electrochemiluminescence sensor provided by the invention can be used to detect chloramphenicol, and has high detection sensitivity and good selectivity.

Description

technical field [0001] The invention relates to the field of antibiotic detection, in particular to a chloramphenicol molecularly imprinted electrochemiluminescent sensor and a method for detecting chloramphenicol. Background technique [0002] Chloramphenicol is a bacteriostatic broad-spectrum antibiotic produced by Streptomyces venezuela, which can effectively kill or inhibit various Gram-positive bacteria and negative bacteria. In recent years, chloramphenicol has been widely used in the treatment of human and animal infectious diseases, and is often added to feed to promote the growth and development of animals, resulting in chloramphenicol residues in food. Long-term use of chloramphenicol-containing medicines or foods containing chloramphenicol will have very toxic side effects on the human body, and even cause serious diseases, such as gray baby syndrome, leukemia and aplastic anemia. Therefore, detecting the content of chloramphenicol in food is of great significanc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/26
Inventor 刘红云廉文静
Owner BEIJING NORMAL UNIVERSITY